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| Comparative transcriptome analysis of molecular regulation mechanism of lipid and starch accumulation in microalgae Ankistrodesmus sp. HJ12 under nitrogen limitation |
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| DOI： |
| KeyWord:microalgae lipid starch transcriptome molecular regulation mechanism |
| FundProject: |
| Author Name | Affiliation | | LIN Weize1,2, MA Shuai2, LI Yajun2, ZHANG Jiaming2,TAN Deguan2, DENG Ruru2, LIU Pinghuai1 | 1.School of Chemical Engineering and Technology, Hainan University, Haikou 570228, China
2.Hainan Key Laboratory of Tropical
Microbe Resources, Hainan Academy of Tropical Agricultural Resources of Chinese Academy of Tropical Agricultural Sciences (CATAS),
Institute of Tropical Bioscience and Biotechnology of CATAS, Haikou 571101, China |
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| Abstract: |
| In order to clarify the relationship of lipid and starch metabolism in microalgae, with Ankistrodesmus sp. HJ12 as the experimental material, its biomass, lipid contents and starch contents were measured under different nitrogen concentrations, and comparative transcriptome were studied. The correlation between microalgae lipid and starch accumulation was explored and their molecular regulation mechanisms were investigated. The results showed that nitrogen concentration remarkedly influenced lipid and starch accumulation in Ankistrodesmus sp. HJ12, and there was a negative correlation between them. The lipid contents of algal cells cultured in media with nitrogen were significantly higher than those in nitrogen-free media. The optimal nitrogen concentration for lipid accumulation was 7.6 mmol/L. In contrast, the starch contents of algal cells were the highest in nitrogen-free media. Comparative transcriptome analysis showed that genes involved in fatty acid synthesis, glycolytic and amylolytic pathway were expressed at higher levels in Ankistrodesmus sp. HJ12 than the control, implying that the cells accumulated more acetyl-CoA and carbon source for fatty acid synthesis under nitrogen limitation, and promoting the synthesis of fatty acids. Moreover, four key genes of regulating lipids anabolism, ACACA, α-AMY, PDC and adh, were selected for RT-qPCR analysis, and the results of gene expression partterns were in consistent with those obtain by transcriptome analysis. The results of the study provided a basis to deeply understand the molecular mechanism of microalgae lipids anabolism. In addition, it added a new favorable foundation for accelerating producing biodiesel from microalgae lipids to achieve large-scale applications. |
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