Optimization of ultrasonic-microwave assisted extraction of selenium-enriched rice bran protein and evaluation of its antioxidant activity
  
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KeyWord:selenium-enriched rice bran  ultrasound-microwave assisted extraction  rice bran protein  antioxidant activity
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Author NameAffiliation
HE Yuanrui1, XIE Yong1, CHEN Zhaojun1, LIU Xiong1,2, WANG Qun3 1.College of Food Science, Southwest University, Chongqing 400715, China
2.Chongqing the West Food Industry Technology Research Institute, Chongqing 402260, China
3.Chongqing Guoke Food Testing Co., Ltd., Chongqing 402260, China 
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Abstract:
      In order to solve the problems of low extraction rate, long extraction time and decreasing functional properties of selenium-enriched rice bran protein(Se-RBP) after extraction, using defatted selenium-enriched rice bran powder as raw material, the process of ultrasonic-microwave assisted extraction(UMAE) of Se-RBP was optimized through single factor experiment and orthogonal experiment, and the extraction effect, antioxidant activity of Se-RBP extracted by UMAE and surface morphology changes of rice bran particles after UMAE were compared with ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and alkali-assisted extraction (AAE). The results showed that the optimal conditions of UMAE was ultrasonic power 600 W, microwave power 500 W, extraction time 20 min and material liquid ratio 1∶40. Under these conditions, the extraction rate of Se-RBP was 86.94%, which increased by 40.96%, 48.00% and 53.50% compared with UAE, MAE and AAE, respectively, and the antioxidant capacity and selenium content of the extracted Se-RBP were higher. The results of scanning electron microscopy showed that the surface of rice bran particles after UMAE was loosened and curled, and the intact cortex structure disappeared, indicating that UMAE could increase the extraction rate of Se-RBP by strongly destroying the structure of rice bran particles, and improve its antioxidant activity. The UMAE technology can promote the extraction of Se-RBP and improve its antioxidant activity.
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