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| Optimization of medium composition for campesterol production in Yarrowia lipolytica by response surface methodology |
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| DOI： |
| KeyWord:Yarrowia lipolytica campsterol response surface methodology medium optimization |
| FundProject: |
| Author Name | Affiliation | | TAN Siyuan1,2, CHEN Yuexing1,2, LEI Qiuyan1, SHE Jiayi1, MENG Yonghong3 | 1.School of Biomedicine and Food Engineering, Shangluo University, Shangluo 726000,Shaanxi, China
2. Shaanxi Qinling Industrial Technology Research Institute of Special Biological Resources, Shangluo 726000, Shaanxi, China
3.College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi′an 710119, China |
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| Abstract: |
| In order to increase the yield of campesterol in Yarrowia lipolytica PMEDD, the fermentation medium composition was optimized. The influences of carbon source type, nitrogen source type, carbon source mass concentration, nitrogen source mass concentration, and KH2PO4 mass concentration on the yield of campesterol were explored by single factor experiment. Then the fermentation medium composition was optimized by response surface methodology. The results showed that the optimal medium composition was obained as follows: glucose 3.17 g/100 mL, peptone 1.44 g/100 mL, KH2PO4 0.65 g/100 mL, Uracil 0.1 g/100 mL, YNB 0.42 g/100 mL, MgSO4·7H2O 0.15 g/100 mL. Under these conditions, the maximal campesterol production of 16.49 μg/mL was obtained, which was 2.10 times of the initial fermentation medium. The optimized medium can effectively increase the yield of campesterol in Yarrowia lipolytica PMEDD. |
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