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| Enzymatic construction of human milk fat substitutes based on human milk fat composition |
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| DOI： |
| KeyWord:human milk fat substitutes construction Chinese human milk fat fatty acid composition structured triglycerides |
| FundProject: |
| Author Name | Affiliation | | ZENG Guibing1,2, WAN Dongman2，3, LUO Miao2，3, ZENG Zheling1,2, YU Ping1,2 | 1.School of Resource & Environmental and Chemical Engineering, Nanchang University, Nanchang 330031, China
2.State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
3.School of Food Science and Technology, Nanchang University, Nanchang 330047, China |
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| Abstract: |
| For the development of new high-similarity human milk fat substitutes， based on the fatty acid composition and triglyceride structure of Chinese human milk fat, five kinds of oils, including basha fish oil, Cinnamomum camphora seed kernel oil, linseed oil, microbial oil(rich in ARA), DHA algae oil, with mass ratio of 153.81∶15.16∶62.33∶7.89∶10.81 were used to prepare the human milk fat substitutes (HMFS) catalyzed by immobilized lipase Lipozyme RM at 60℃ for 8 h，and its fatty acid, sn-2 fatty acid and triglyceride compositions and contents were determined, as well as the infrared spectra, melting properties, crystalline properties, and rheological properties. The results showed that the HMFS contained 12% of medium-chain fatty acids, 19.53% of linoleic acid-linolenic acid with ratio 1∶1.5, 1.17% of ARA and 1.11% of DHA, and its sn-2 position had high content of palmitic acid (43.10%), and it had high content of structured triglycerides(58.75%)，and no trans fatty acid were formed. The melting temperature of HMFS was below body temperature and the solid fat content of HMFS at 20℃ was 18.7%. In addition, the crystals form of HMFS were fine and uniform, the rheological properties of HMFS were excellent. The HMFS obtained has potential application in various infant foods such as infant formula, infant rice cereal and infant biscuits. |
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