| In order to improve the stability and water solubility of lycopene, lycopene microemulsion was prepared by low energy emulsification method. The formulation of lycopene microemulsion was optimized by pseudo-ternary phase diagram.The influences of oil phase,emulsifier,assistant emulsifier,mass ratio of emulsifier to assistant emulsifier(Km value)on the formation of microemulsion were discussed.The optimal value of each factor was determined by calculating and comparing the microemulsion area of the pseudo-ternary phase diagram.The type, morphology, particle size, polydispersity index and Zeta potential of lycopene microemulsion prepared with the optimal formula were measured. The results showed that the best formula of lycopene microemulsion was:oil phase was linseed oil,emulsifier Twain 80, assistant emulsifier anhydrous ethanol, Km value 2∶1. Using the best formula, the lycopene microemulsion was O/W type microemulsion in regular spherical shape, and its particle size was (167.70±0.45) nm,the PDI was 0.179±0.006,and the Zeta potential was (-7.71±0.53)mV. The prepared lycopene microemulsion was homogeneous and stable. |
