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| Enzymatic preparation process optimization and physicochemical properties analysis of giant salamander liver oil |
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| DOI： |
| KeyWord:giant salamander liver oil enzymatic extraction process response surface methodology physicochemical property fatty acid |
| FundProject: |
| Author Name | Affiliation | | CHEN Yanjie1, TANG Jiacheng1, GONG Xuan1, HUANG Kecheng1,SHAN Qianyi2, BAO Jianqiang1,3,4 | 1.College of Food Science, Shanghai Ocean University, Shanghai 201306, China
2.National Food Quality Supervision and Inspection Center, Shanghai Institute of Quality Supervision and Inspection Technology,Shanghai 200233, China
3.Shanghai Engineering Research Center of Aquatic Product Processing & Preservation, Shanghai 201306, China
4.Ministry of Agriculture Laboratory for Quality, Safety and Risk Assessment of Storage and Preservation of Aquatic Products(Shanghai), Shanghai 201306, China |
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| Abstract: |
| In order to improve the comprehensive utilization value of the waste material of giant salamander, the oil was extracted from liver of artificial cultured giant salamander by enzymatic hydrolysis method. The protease was screened, and the influences of pH, liquid-solid ratio, enzyme dosage, enzymolysis time and enzymolysis temperature on the extraction rate of giant salamander liver oil were investigated by single factor experiment. On this basis, the giant salamander liver oil extraction process conditions were optimized by response surface methodology. The physicochemical indicators and fatty acid composition of the giant salamander liver oil before and after refining were determined. The results showed that the optimal conditions of extracting oil from giant salamander liver were obtained as follows:using papain, pH 6.5, liquid-solid ratio 2.5∶ 1, enzyme dosage 2 100 U/g, enzymolysis time 4 h, and enzymolysis temperature 65℃. Under these conditions, the extraction rate of giant salamander liver oil was 79.92%. The acid value, peroxide value, iodine value and content of unsaturated fatty acid of the crude giant salamander liver oil were 5.73 mgKOH/g, 1.04 mmol/kg,124.60 gI/100 g and 69.75%, respectively. The acid value, peroxide value and iodine value of the refined giant salamander liver oil were 0.93 mgKOH/g, 0.43 mmol/kg, 129.40 gI/100 g, respectively. A total of 18 fatty acids were detected in refined giant salamander liver oil, and the contents of unsaturated fatty acid and polyunsaturated fatty acid were 73.55%and 33.88%, respectively. Using papain to extract giant salamander liver oil has advantages of mild reaction conditions, simple operation and high extraction rate. The giant salamander liver oil obtained is of good quality, rich in content of unsaturated fatty acids, and has high nutritional value. |
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