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| Effects of freeze-thaw demulsification methods on aqueous extraction of oil-tea camellia seed oil |
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| KeyWord:oil-tea camellia seed oil freeze-thaw demulsification heating pH |
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| Abstract: |
| In order to improve the oil extraction rate of oil-tea camellia seed oil by aqueous extraction method, the oil-tea camellia seed was treated by peeling, crushing, sieving, mixing with water through colloid mill and centrifugation to get the emulsion, and the oil-tea camellia seed oil was obtained by centrifugation after freeze-thaw demulsification. With the oil extraction rate as the index, the effects of freeze-thaw demulsification methods, such as heating freeze-thaw, ethanol freeze-thaw, pH adjustment freeze-thaw, salt freeze-thaw, on the extraction of oil-tea camellia seed oil by aqueous extraction method were studied. The microstructure of the emulsion before and after demulsification was observed by confocal laser scanning microscope. The results showed that ethanol freeze-thaw and salt freeze-thaw reduced the oil extraction rate. The oil extraction rate increased by adjusting pH, and it was 92.17% at pH 8. Heating freeze-thaw was the best method of freeze-thaw demulsification. Under heating temperature 60℃ and heating time 20 min, the oil extraction rate was the highest, reaching 92.57%. After heating freeze-thaw, the oil droplets gathered and its particle size increased significantly. Demulsification of emulsion by heating freeze-thaw method can effectively increase the oil extraction rate of oil-tea camellia seed oil by aqueous extraction method. |
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