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| Optimization of enzymatic hydrolysis preparation of Korean pine kernel polypeptide and analysis of its antihypertensive and lipid-lowering activities |
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| KeyWord:Korean pine kernel polypeptide enzymatic hydrolysis antihypertensive activity lipid-lowering activity |
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| Abstract: |
| In order to provide reference for the development and application of Korean pine kernel polypeptide, the Korean pine kernel protein was extracted by acid leaching method with Korean pine kernel meal as raw materials, and then the polypeptide was prepared by enzymatic hydrolysis. Taking the degree of hydrolysis as the evaluation index, the single factor experiment and response surface methodology were used to optimize the enzymatic hydrolysis preparation process conditions of Korean pine kernel polypeptide. At the same time, the molecular mass distribution and amino acid composition of the polypeptides prepared under the optimal conditions were analyzed, and the antihypertensive and lipid-lowering activities of the polypeptides were determined. The results showed that the optimal enzymatic hydrolysis process conditions for the Korean pine kernel polypeptide was the step-by-step enzymatic hydrolysis method, and Korean pine kernel protein was hydrolyzed for 2.5 h with papain under the conditions of enzymatic hydrolysis temperature 50℃, substrate mass concentration 6.0 g/100 mL, papain dosage 10 500 U/g, and enzymatic hydrolysis pH 6.0, and then continuously hydrolyzed for 2.3 h with alkaline protease under the conditions of alkaline protease dosage 10 970 U/g and enzymatic hydrolysis pH 9.0. Under the optimal conditions, the degree of hydrolysis of Korean pine kernel protein reached 25.94%. The number average molecular mass of Korean pine kernel polypeptide was 2 272 Da, and the weight-average molecular mass was 4 080 Da. The contents of glutamic acid, arginine and aspartic acid in the Korean pine kernel polypeptide were relatively high, and the content of essential amino acids accounted for 30.23% of the total amino acids. The polypeptides with a molecular mass of less than 10 kDa accounted for 92%, and the polypeptides with a molecular mass of 0.5-1.0 kDa had the highest ACE inhibitory activity, with an IC50 of 66.05 μg/mL. The polypeptides with a molecular mass of 1.0-5.0 kDa had the highest pancreatic lipase inhibitory activity, with an IC50 of 194.43 μg/mL. In conclusion, Korean pine kernel polypeptide has high nutritional value and certain ACE and pancreatic lipase inhibitory activities. |
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