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| 红松仁多肽酶解制备工艺优化及降压降脂活性分析 |
| Optimization of enzymatic hydrolysis preparation of Korean pine kernel polypeptide and analysis of its antihypertensive and lipid-lowering activities |
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| DOI： |
| 中文关键词: 红松仁 多肽 酶解 降压活性 降脂活性 |
| 英文关键词:Korean pine kernel polypeptide enzymatic hydrolysis antihypertensive activity lipid-lowering activity |
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| 中文摘要: |
| 为了对红松仁多肽的开发及应用提供参考，以红松仁粕为原料，采用酸浸提法提取红松仁蛋白，然后采用酶解法制备红松仁多肽。以水解度为评价指标，通过单因素实验和响应面实验对红松仁多肽酶解制备工艺条件进行了优化。同时，对最佳工艺条件下制备的多肽的分子质量分布和氨基酸组成进行分析，并测定了多肽的降压降脂活性。结果表明：红松仁多肽的最佳酶解制备工艺条件为采用分步酶解法，在酶解温度50℃、底物质量浓度6.0 g/100 mL、木瓜蛋白酶添加量10 500 U/g、酶解pH 6.0条件下酶解2.5 h，然后在碱性蛋白酶添加量10 970 U/g、酶解pH 9.0条件下酶解2.3 h，在此工艺条件下红松仁蛋白的水解度达25.94%；红松仁多肽的数均分子质量为2 272 Da，重均分子质量为4 080 Da；红松仁多肽中谷氨酸、精氨酸和天冬氨酸含量较高，必需氨基酸含量占氨基酸总量的 30.23%；红松仁多肽中分子质量小于10 kDa的多肽占92%，分子质量0.5~1.0 kDa的多肽具有最高的ACE抑制活性，IC50为66.05 μg/mL；分子质量1.0~5.0 kDa的多肽具有最高的胰脂肪酶抑制活性，IC50为194.43 μg/mL。综上，红松仁多肽具有较高的营养价值，具有一定的ACE和胰脂肪酶抑制活性。 |
| 英文摘要: |
| In order to provide reference for the development and application of Korean pine kernel polypeptide, the Korean pine kernel protein was extracted by acid leaching method with Korean pine kernel meal as raw materials, and then the polypeptide was prepared by enzymatic hydrolysis. Taking the degree of hydrolysis as the evaluation index, the single factor experiment and response surface methodology were used to optimize the enzymatic hydrolysis preparation process conditions of Korean pine kernel polypeptide. At the same time, the molecular mass distribution and amino acid composition of the polypeptides prepared under the optimal conditions were analyzed, and the antihypertensive and lipid-lowering activities of the polypeptides were determined. The results showed that the optimal enzymatic hydrolysis process conditions for the Korean pine kernel polypeptide was the step-by-step enzymatic hydrolysis method, and Korean pine kernel protein was hydrolyzed for 2.5 h with papain under the conditions of enzymatic hydrolysis temperature 50℃, substrate mass concentration 6.0 g/100 mL, papain dosage 10 500 U/g, and enzymatic hydrolysis pH 6.0, and then continuously hydrolyzed for 2.3 h with alkaline protease under the conditions of alkaline protease dosage 10 970 U/g and enzymatic hydrolysis pH 9.0. Under the optimal conditions, the degree of hydrolysis of Korean pine kernel protein reached 25.94%. The number average molecular mass of Korean pine kernel polypeptide was 2 272 Da, and the weight-average molecular mass was 4 080 Da. The contents of glutamic acid, arginine and aspartic acid in the Korean pine kernel polypeptide were relatively high, and the content of essential amino acids accounted for 30.23% of the total amino acids. The polypeptides with a molecular mass of less than 10 kDa accounted for 92%, and the polypeptides with a molecular mass of 0.5-1.0 kDa had the highest ACE inhibitory activity, with an IC50 of 66.05 μg/mL. The polypeptides with a molecular mass of 1.0-5.0 kDa had the highest pancreatic lipase inhibitory activity, with an IC50 of 194.43 μg/mL. In conclusion, Korean pine kernel polypeptide has high nutritional value and certain ACE and pancreatic lipase inhibitory activities. |
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