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| Determination of aflatoxin B1 in finished vegetable oil and crude peanut oil by multifunctional clean-up column-photochemical derivatization and high performance liquid chromatography |
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| DOI： |
| KeyWord:aflatoxin B1 multifunctional clean-up column vegetable oil high performance liquid chromatography |
| FundProject: |
| Author Name | Affiliation | | JIANG Deming1, LIU Xiaomeng1, ZOU Qiulong1, YIN Tie1, ZHANG Xiaolin1, ZHANG Gang2, LIU Peilian2 | 1.COFCO Nutrition & Health Research Institute, Beijing 102209, China
2.COFCO Oil and Grains Industry (Feixian) Co. , Ltd. , Linyi 273400, Shandong, China |
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| Abstract: |
| To determine aflatoxin B1(AFB1) in finished vegetable oil and crude peanut oil quickly and accurately,a method was developed for the determination of AFB1 content in vegetable oil using a multifunctional clean-up column for the pretreatment of oil samples combined with photochemical derivatization and high performance liquid chromatography(HPLC). The pretreatment extractant and the HPLC analysis conditions (chromatographic column and injection volume) were optimized,and the method was evaluated by comparing with the national standard of immune- affinity column. The results showed that the optimal conditions were with acetonitrile-water solution (84+16) as the extractant, Zorbax Eclipse XDB-C18 (4.6 mm×150 mm， 5 μm) chromatographic column and injection volume 10 μL. The established method had good spiked recovery and precision,and the quantitation limit was 0.2 μg/kg, lower than the minimum limit specified in GB 2761-2017, which could meet the requirements of actual production. When the established method was used for the determination of AFB1 in light-colored vegetable oils, the relative error of the detection results compared with the immune-affinity column method was 0-18.0%, which was in accordance with the national standard, but the relative error for the determination of dark-colored vegetable oils exceeded the national standard. In summary, the method can detect AFB1 content in light vegetable oil such as peanut oil (finished oil and crude oil) and finished corn oil, flaxseed oil, sunflower seed oil, light yellow rapeseed oil and yellow sesame oil quickly and accurately. |
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