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| Hydrolysis of soybean isoflavones catalyzed by cellulase |
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| DOI： |
| KeyWord:soybean isoflavone hydrolysis rate of glycosides conversion rate of aglycones cellulase |
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| Abstract: |
| In order to improve the biological-value of soybean isoflavones, the glycoside-type soybean isoflavones was hydrolyzed to prepare free aglycon-type soybean isoflavones with cellulase as catalyst. A low-cost and effective cellulase was selected by investigating the effects of 10 cellulases on the total hydrolysis rate of glycoside-type soybean isoflavones and the total conversion rate of aglycone-type soybean isoflavones. Meanwhile, the cellulase selected was used to catalyze the hydrolysis of glycoside-type soybean isoflavones, and the parameters such as the substrate mass concentration, enzyme amount, reaction system pH, enzymatic temperature and enzymatic time were optimized. The results showed that the cellulase from Trichoderma viride was selected as the enzyme for the hydrolysis of soybean isoflavones, and the more economical and efficient hydrolysis parameters were obtained as follows: substrate mass concentration 0.8-2.0 mg/mL, enzyme amount 7%-11%, reaction system pH 5.0, hydrolysis temperature 55℃, and hydrolysis time 5-6 h. In the optimization, the total hydrolysis rate of glycoside-type soybean isoflavones exceeded 90%, and the total conversion rate of aglycone-type soybean isoflavones was close to 60%. Therefore, the use of cellulase-catalyzed hydrolysis of soybean isoflavones can significantly increase the content of free aglycones and improve the utilization value of soybean isoflavones. |
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