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| 利用酒糟处理液作为氮源培养裂殖壶菌生产DHA的发酵工艺优化 |
| Optimization of fermentation process of culturing Schizochytrium to produce DHA using distiller′s grains treatment liquid as nitrogen source |
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| DOI： |
| 中文关键词: 酒糟处理液 裂殖壶菌 DHA 发酵废液 |
| 英文关键词:distiller′s grains treatment liquid Schizochytrium DHA fermentation wastewater |
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| 中文摘要: |
| 为降低发酵培养裂殖壶菌生产DHA的成本，以酱香型白酒酒糟为实验材料，将其简单预处理后作为裂殖壶菌发酵培养基氮源，首先分析了酒糟处理液与胰蛋白胨的游离氨基酸组成与含量，然后以生物量、油脂产量和DHA产量为指标，对酒糟处理液、胰蛋白胨、酵母提取物和牛肉膏作为氮源培养裂殖壶菌生产DHA进行了比较，优化了酒糟处理液作为氮源时的发酵工艺条件，并对发酵废液进行三次循环利用。结果表明：酒糟处理液可以作为满足裂殖壶菌生长的氮源；与酵母提取物、牛肉膏相比，酒糟处理液作为氮源有明显优势；利用酒糟处理液作为氮源的最佳发酵工艺条件为酒糟（含水量约10%）与水质量比1∶ 9、酒糟处理液添加量70%（与基础发酵培养基中水的置换比例）、初始pH 7.0、培养时间108 h,在此条件下裂殖壶菌生物量、油脂产量和DHA产量分别达到22.14、8.88 g/L和284 g/L；最佳条件下三次循环添加15.0%（与基础发酵培养基中水的置换比例）发酵废液于酒糟处理液培养基中，裂殖壶菌DHA产量分别为4.27、3.70 g/L和2.75 g/L。综上，利用酒糟处理液培养裂殖壶菌生产DHA是酒糟资源化利用的新方法。 |
| 英文摘要: |
| In order to reduce the cost of producing DHA by fermentation culture of Schizochytrium, the sauce-flavor distiller′s grains were used as the experimental material, and it was simply pretreated as the nitrogen source of the fermentation culture medium of Schizochytrium.Firstly, the free amino acid composition and content of distiller′s grains treatment liquid and tryptone were analyzed. Then, the biomass, oil yield and DHA yield were used as indicators, the production of DHA by Schizochytrium using distiller′s grains treatment liquid, tryptone, yeast extract and beef extract as nitrogen sources was compared, and the fermentation conditions were optimized when distiller′s grains treatment liquid was used as nitrogen source.The fermentation wastewater was recycled for three times. The results showed that the distiller′s grains treatment liquid could be used as a nitrogen source for the growth of Schizochytrium. Compared with yeast extract and beef extract, distiller′s grains treatment liquid had obvious advantages as nitrogen source. The optimal fermentation conditions for using distiller′ s grains treatment liquid as nitrogen source were obtained as follows: mass ratio of distiller′s grains (about 10% of water content) to water 1∶ 9, dosage of distiller′s grains treatment liquid 70%(replacement ratio of water in the basic fermentation culture medium), initial pH 70, and incubation time 108 h.Under these conditions, the biomass, oil yield, and DHA yield of Schizochytrium reached 22.14, 8.88 g/L, and 2.84 g/L, respectively. After three cycles of adding 15.0% (replacement ratio of water in the basic fermentation culture medium) of fermentation wastewater into the distiller′s grains treatment liquid culture medium, the DHA yield of Schizochytrium was 4.27, 3.70, 2.75 g/L respectively. In summary, it is a new method of resource utilization of distiller′s grains to cultivate Schizochytrium to produce DHA by distiller′s grains treatment liquid. |
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