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| Response surface optimization of solid phase microextraction condition for the determination of volatiles in Fengdan seed oil by SPME-GC-MS |
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| DOI： |
| KeyWord:Fengdan seed oil volatile solid phase microextraction response surface optimization gas chromatography-mass spectrometry |
| FundProject: |
| Author Name | Affiliation | | SHUAI Jiaqi1, LYU Changping2,3, YANG Zhengmin1, SHI Yang1, MAO Mi1, JIANG Lina1, KANG Min1 | 1.College of Horticulture, Hunan Agricultural University, Changsha 410128,China
2.School of Landscape Architecture and Art Design, Hunan Agricultural University, Changsha 410128, China
3.Engineering Technology Research Center for High Quality Flowers and Trees Breeding and Utilization in Central Subtropical Zone of Hunan Province, Hunan Agricultural University, Changsha 410128, China |
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| Abstract: |
| In oder to provide reference for the study of flavor of Fengdan seed oil, the volatile composition of Fengdan seed oil was determined by solid-phase microextraction combined with gas chromatography-mass spectrometry (SPME-GC-MS). On the basis of the single factor experiment, according to the Box-Behnken experimental design principle, with the total peak area of volatile in Fengdan seed oil as the response value, the response surface methodology was conducted to optimize the conditions for the solid-phase microextraction of volatiles in Fengdan seed oil, with the sample amount, equilibrium time, extraction time, and extraction temperature as the factors. Then the volatile composition and relative content of Fengdan seed oil were determined by SPME-GC-MS. The results showed that the optimal conditions for solid phase microextraction of the volatiles in Fengdan seed oil were obtained as follows: sample amount 4.0 g(15 mL sample flask), equilibrium time 40 min, extraction time 20 min, and extraction temperature 80℃. Under the optimal conditions and combined with GC-MS analysis, 22 kinds of volatiles in Fengdan seed oil were detected. Among them, the relative contents of alkenes, esters, aldehydes, alcohols, heterocyclics, aromatic hydrocarbon and phenols were 33.34%, 21.12%, 20.76%, 19.05%, 3.48%, 1.67% and 0.61%, respectively. In conclusion, the solid phase microextraction conditions optimized by the response surface methodology are suitable for rapid determination of volatile in Fengdan seed oil, and alkenes, esters, aldehydes and alcohols are important volatile compounds in Fengdan seed oil. |
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