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| Determination and analysis of heterocyclic aromatic amines in sesame oil extracted from sesame of different producing areas |
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| KeyWord:heterocyclic aromatic amines sesame oil norharman harman determination |
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| Abstract: |
| Sesame oil may contain the heterocyclic aromatic amines (HAAs) 9H-pyrido\[3,4-b\]indole (norharman) and 1-methyl-9H-pyrido\[3,4-b\]indole (harman). A high performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for effectively detecting HAAs content in sesame oil and used to compare the HAAs content difference among sesame oils extracted from sesame of different producing areas. The results showed that HPLC-FLD could complete determination for HAAs in sesame oils within 15 min. The content of HAAs in 14 sesame oils from different producing areas were significantly different (p<0.05) and the HAAs in sesame oil from Africa (421.87 μg/kg) was higher than that from Asia (329.32 μg/kg) under the same roasting conditions. Among the 14 sesame oils, the highest HAAs (720.81 μg/kg) was found in sesame oil from Ethiopia, and the lowest HAAs (236.01 μg/kg and 239.63 μg/kg) was found in sesame oil from Sudan and Zhumadian, Henan province, China, respectively. In conclusion, the content of HAAs in sesame oil is related to the sesame raw materials, and the selection of suitable sesame raw materials is important for the reduction and control of HAAs in sesame oil and for the safety of sesame oil consumption. |
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