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Effect of heterologous expression of fasA gene of Corynebacterium glutamicum fatty acid synthase on fatty acid synthesis
  
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KeyWord:fatty acid synthase  Corynebacterium glutamicum  palmitoleic acid  oleic acid  heterologous expression
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JIN Jingjing，SUN Ziyu，MAN Dula，LIU Jin，HU Chenyue，CHEN Zhongjun College of Food Science and Engineering,Inner Mongolia Agricultural University，Hohhot 010018，China 
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Abstract:
      In order to lay a theoretical foundation for screening and constructing fatty acid high-yielding strains, the effects of Corynebacterium glutamicum fatty acid synthase A (FasA) on the synthesis of fatty acids in E. coli were studied, the properties of FasA were predicted and analyzed using bioinformatics software, moreover the overexpression vector pXMJ19-fasA was constructed and transformed into E. coli for expression, the concentration of expression inducer isopropyl-β-D-thiogalactoside (IPTG) was investigated and the fatty acid composition after heterologous expression was determined.The results showed that when the recombinant strain was induced under the IPTG concentration of 1 mmol/L for 20 h，the yield of palmitoleic acid and oleic acid reached 1.735，2.325 mg/g ，which increased by 53.95% and 59.68% respectively compared with the control strain. These results indicate that FasA can promote the synthesis of palmitoleic acid and oleic acid by E. coli.
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