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| Preparation and component, structure analysis of purified polysaccharides from Camellia oleifera fruit hull |
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| DOI： |
| KeyWord:Camellia oleifera fruit hull polysaccharides monosaccharide composition structure purification |
| FundProject: |
| Author Name | Affiliation | | SHU Binbin1,2,3, WU Suxi1,2,3, XIE Yanyi1，YUAN Chenghua2，FENG Zongyi3 | 1.College of Food and Bio-Engineering, Changsha University of Science and Technology, Changsha 410114, China
2.Hunan Kangduoli Oil Co. , Ltd. ,Changde 415700,Hunan, China
3.Hunan Yamei Biology Science and Technology Co. , Ltd. , Zhuzhou 412300,Hunan,China |
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| Hits: 2020 |
| Download times: 2295 |
| Abstract: |
| In order to develop and utilize Camellia oleifera fruit hull resources with high value, the crude polysaccharide was extracted by water extraction-alcohol precipitation process with Camellia oleifera fruit hull as raw material, and it was purified by sodium type 732 cation adsorption resin to obtain purified polysaccharide of Camellia oleifera fruit hull. The purity of the eluent component polysaccharide was determined by ultraviolet spectroscopy, the monosaccharide composition of purified polysaccharide of Camellia oleifera fruit hull was analyzed by ion chromatography, the main chain structure and functional groups of the purified polysaccharide was analyzed by infrared spectroscopy, and the surface structure characteristics of the purified polysaccharide was analyzed by scanning electron microscopy. The results showed that the polysaccharide content of Camellia oleifera fruit hull was 11.38%. The purity of purified polysaccharide of Camellia oleifera fruit hull was 87.40% and the yield was 2.33%. The monosaccharide composition of the purified polysaccharide of Camellia oleifera fruit hull was fucose 0.64%, arabinose 7.68%, galactose 3.74%, glucose 76.43%, xylose 2.77%, mannose 1.60%, galacturonic acid 5.34%, glucuronic acid 1.33%, and mannuronic acid 0.47%. The purified polysaccharide obtained from Camellia oleifera fruit hull was a kind of acidic polysaccharide with β-type pyranose as main chain, and its surface was tight and slightly smooth with holes and bubble particles. In summary, after water extraction, alcohol precipitation and column chromatography purification, the polysaccharide purity of Camellia oleifera fruit hull gradually increased, which could provide a theoretical basis for the follow-up structure-activity relationship exploration and industrial development of Camellia oleifera fruit hull. |
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