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| Pretreatment for determination of total triterpene in oil-tea camellia seed oil by spectrophotometry |
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| DOI： |
| KeyWord:oil-tea camellia seed oil total triterpene spectrophotometry pretreatment |
| FundProject: |
| Author Name | Affiliation | | YE Meijin1,2, YANG Yumin3, FENG Hong1,2, JIANG Hongyu1,2, HE Ziting1, QIU Yufan1 | 1.College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu 611130, China
2.Key Laboratory of Development and Utilization of Characteristic Horticultural Plant Resources,Sichuan Provincial Higher University, Chengdu 611130, China
3.Institute of Agricultural Resources and Environment, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China |
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| Abstract: |
| To accurately quantify triterpenoids in oil-tea camellia seed oil, a sample pretreament method was investigated for the determination of total triterpene in oil-tea camellia seed oil by spectrophotometry. The effects of volume fraction of ethanol, extraction temperature, extraction time and extraction times on the extraction of total triterpene from oil-tea camellia seed oil were investigated by single factor experiment and orthogonal experiment. The results showed that the significance of the factors affecting the extraction of total triterpene from oil-tea camellia seed oil decreased in the order: ethanol volume fraction> extraction times> extraction temperature = extraction time. The optimal extraction conditions of total triterpene were as follows: volume fraction of ethanol 80%, extraction temperature 80℃, extraction time 40 min, and extraction times 4. Under these conditions, the content of total triterpene in oil-tea camellia seed oil was (0.78±0.06)% (calculated by oleanolic acid). In conclusion, the method is simple, rapid and sensitive, and can be used for the determination of total triterpene in oil-tea camellia seed oil. |
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