| In order to improve work efficiency and reduce errors, a molecularly imprinted column automated solid phase extraction-high performance liquid chromatography method for determination of benzo(a)pyrene content in edible vegetable oil was established. The benzo(a)pyrene in samples was extracted using n-hexane, purified by automated solid phase extraction instrument with molecularly imprinted column, separated by C18 column and detected by high performance liquid chromatography with a fluorescence detector. The results showed that benzo (a) pyrene had a good linear relationship with peak area in the range of 0.5-20.0 ng/mL, with a correlation coefficient of 0.999 9. The limit of detection was 0.09 μg/kg, and the limit of quantitation was 0.30 μg/kg. The average recovery rate was 91.7%-111.8% with the relative standard deviation of 2.0%-8.2%. In the detection of edible vegetable oil, there was no significant difference between the established method and the national standard method.Afterwards, the method was applied to determine the benzo(a)pyrene content in commercial 38 edible vegetable oil samples, and the detection rate was 76.3%. In conclusion, the method is highly accurate and repeatable, and can improve the efficiency using batch automation preprocessing, reduce the risk of personnel operation error, and is suitable for the measurement of benzo(a) pyrene content in edible vegetable oil. |
