| In order to improve the utilization of Cucumis melo L.seeds, Cucumis melo L.seed oil was extracted by ultrasonic-assisted extraction.The yield of Cucumis melo L.seed oil and DPPH radical scavenging rate were taken as indicators, and the effects of ultrasonic time, ultrasonic temperature, solid-liquid ratio, and extraction time on the extraction of Cucumis melo L.seed oil were investigated by single factor experiment,then the extraction conditions were optimized by response surface methodology.The physicochemical indexes, total phenol content, composition and contents of sterol and fatty acid of extracted Cucumis melo L.seed oil were determined.The results showed that the optimal extraction conditions were obtained as follows: ultrasonic time 41 min,ultrasonic temperature 43 ℃,solid-liquid ratio 1∶ 7.7, and extraction time 3 h. Under these conditions, the yield of Cucumis melo L.seed oil was 25.64%, and the DPPH radical scavenging rate was 60.66%. The acid value,peroxide value, iodine value and total phenol content of Cucumis melo L.seed oil were 0.52 mgKOH/g, 0.00 mmol/kg，133.38 gI/100 g, and 8.72 mg/100 g，respectively.A total of five fatty acids were detected in Cucumis melo L. seed oil, of which linoleic acid had the highest content of 66.68%, and seven sterols were detected, with a total content of 306.10 mg/100 g, of which β-sitosterol had the highest content of 138.60 mg/100 g. Cucumis melo L.seed oil has high nutritional value and can be further developed and utilized as an edible oil. |
