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| 水杨酸诱导对斜生栅藻生长及油脂积累的影响 |
| Effects of salicylic acid induction on growth and lipid accumulation of Scenedesmus obliquus |
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| DOI： |
| 中文关键词: 水杨酸 斜生栅藻 叶绿素a 油脂 官能团 |
| 英文关键词:salicylic acid Scenedesmus obliquus chlorophyll a lipid functional group |
| 基金项目:国家自然科学基金项目（52170045）；山西省自然科学基金(201901D211029) |
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| 中文摘要: |
| 为缓解化石能源压力，提高生物质能源的使用率，以空气中CO2作为自养培养斜生栅藻的碳源，添加植物激素水杨酸（SA）诱导斜生栅藻，研究斜生栅藻细胞密度、叶绿素a含量、油脂含量及产率等的变化情况，探讨在SA诱导下斜生栅藻的产脂机制。结果表明：培养16 d，SA对斜生栅藻生长及油脂积累表现出显著促进效果，当SA质量浓度为20 mg/L时，细胞密度最大，为（1.89±006）g/L，较对照组提高了34.47%；在实验范围内（SA质量浓度0～40 mg/L），油脂产率与SA质量浓度呈线性正相关；油脂含量及产率均在SA质量浓度为40 mg/L时达到最大，分别为（48.49±7.47）%和（22.35±3.77）mg/（L·d），较对照组分别提高了72.85%和1.43倍；SA对斜生栅藻细胞官能团中的酯基、羧基、磷酸基团等产生了影响；同时，在SA诱导下斜生栅藻的油脂代谢途径与叶绿素、酶、抗逆性等因素有关。综上，SA既提高了斜生栅藻的生物量，又促进了其光合作用和储能物质转化，提高了油脂产率。 |
| 英文摘要: |
| In order to alleviate the pressure of fossil energy, and improve the utilization rate of biomass energy, Scenedesmus obliquus（S. obliquus）was autotrophic cultivated by using CO2 in the air as the carbon source and adding the phytohormone salicylic acid（SA）. The changes of cell density, chlorophyll a content, lipid content and productivity, and the mechanism of lipid production of S.obliquus were investigated. The results showed that SA had a significant effect on promoting the growth and lipid accumulation of S. obliquus after 16 d of culture. When the SA mass concentration was 20 mg/L, the cell density reached a maximum of (1.89±0.06) g/L, which was 34.47% higher than that of the control group. In the experimental mass concentration range (0-40 mg/L), the productivity of lipid was positively correlated with the mass concentration of SA. The lipid content and productivity reached the maximum at 40 mg/L with (48.49±7.47)% and (22.35±3.77)mg/(L·d) respectively, which were 72.85% and 1.43 times higher than that of the control group. SA had effects on ester group, carboxyl group, phosphate group, etc. in the functional groups of S. obliquus cells. At the same time, the lipid metabolism pathway of S. obliquus induced by SA was related to chlorophyll, enzymes, stress resistance and other factors. In conclusion, SA can increase the biomass of S. obliquus, promote the photosynthesis and energy storage material conversion of S. obliquus, and improve the lipid productivity. |
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