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Effects of salicylic acid induction on growth and lipid accumulation of Scenedesmus obliquus |
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DOI: |
KeyWord:salicylic acid Scenedesmus obliquus chlorophyll a lipid functional group |
FundProject:国家自然科学基金项目(52170045);山西省自然科学基金(201901D211029) |
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Abstract: |
In order to alleviate the pressure of fossil energy, and improve the utilization rate of biomass energy, Scenedesmus obliquus(S. obliquus)was autotrophic cultivated by using CO2 in the air as the carbon source and adding the phytohormone salicylic acid(SA). The changes of cell density, chlorophyll a content, lipid content and productivity, and the mechanism of lipid production of S.obliquus were investigated. The results showed that SA had a significant effect on promoting the growth and lipid accumulation of S. obliquus after 16 d of culture. When the SA mass concentration was 20 mg/L, the cell density reached a maximum of (1.89±0.06) g/L, which was 34.47% higher than that of the control group. In the experimental mass concentration range (0-40 mg/L), the productivity of lipid was positively correlated with the mass concentration of SA. The lipid content and productivity reached the maximum at 40 mg/L with (48.49±7.47)% and (22.35±3.77)mg/(L·d) respectively, which were 72.85% and 1.43 times higher than that of the control group. SA had effects on ester group, carboxyl group, phosphate group, etc. in the functional groups of S. obliquus cells. At the same time, the lipid metabolism pathway of S. obliquus induced by SA was related to chlorophyll, enzymes, stress resistance and other factors. In conclusion, SA can increase the biomass of S. obliquus, promote the photosynthesis and energy storage material conversion of S. obliquus, and improve the lipid productivity. |
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