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| Optimization of the preparation process of red kernel walnut polypeptide and its hypolipidemic effect |
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| DOI： |
| KeyWord:red kernel walnut protein isolate enzymatic hydrolysis polypeptide hypolipidemic |
| FundProject:陕西省林业科学院科技创新计划项目（SXLK2021-0232）;2020年度陕西省农业产业化发展项目；陕西省农业领域重点产业链项目（2021ZDLNY04-05） |
| Author Name | Affiliation | | WU Shujuan1, WANG Rui2, DONG Zhaobin3, XIA Yucheng1,
LIAO Yumei4, ZHANG Runguang1, ZHANG Youlin1 | 1.College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi′an 710119, China
2.Shaanxi Forestry Science and Technology Promotion and International Project Management Center, Xi′an
710082, China
3.Shaanxi Shangluo Shengda Industrial Corporation Limited, Shangluo 726000, Shaanxi, China
4.Shaanxi Chenggu County Agricultural Machinery Management Station, Hanzhong 723200, Shaanxi, China |
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| Abstract: |
| In order to provide reference for the deep processing and health function of red kernel walnut, the red kernel walnut polypeptide was prepared by the enzymatic hydrolysis of red kernel walnut protein isolate, the single factor test and orthogonal test were used to optimize the enzymolysis process conditions. Three components of red kernel walnut polypeptide were separated by ultrafiltration, and its polypeptide content, the inhibition rates of pancreatic lipase and cholesterol micelle solubility were measured. Taking hyperlipidemia SD rats as a model, and low, medium, and high doses (200,400,800 mg/kg) of red kernel walnut polypeptide with the highest activity were administered to the rats for 28 d, and the body weight, organ weight, serum biochemical indicators of rats were measured and the tissue sections were observed. The results showed that the optimal conditions for the preparation of red kernel walnut polypeptide were as follows: enzymatic pH 9.0, mass fraction of substrate 4.0%, enzyme addition amount (calculated by the total mass of the reaction system) 4.0%, and enzymatic temperature 55 ℃. Under these conditions, the degree of hydrolysis was 6.96%. Components with molecular mass less than 3 kDa had the highest activity. Red kernel walnut polypeptide gavage significantly reduced the increase of body weight and mass of liver, kidney and epididymal adipose tissue in SD rats(p<0.05), and serum total cholesterol, triglycerides, low-density lipoprotein cholesterol and arterisclerosis index were significantly reduced in rats, while the high-density lipoprotein cholesterol content was increased (p<0.05,p<0.01). The tissue section observation showed that the intervention of red walnut polypeptide could inhibit the growth and accumulation of adipose tissue. The hydrolyzed polypeptides of red kernel walnut protein isolate have a good hypolipidemic effect. |
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