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| TGase-induced zein to improve properties of soy protein isolates film |
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| DOI： |
| KeyWord:soy protein isolates zein transglutaminase film cross-linking |
| FundProject:黑龙江省“百千万”工程科技重大专项（2021ZX12B09）；2020年度黑龙江省玉米主食工业化工程技术研究中心开放课题（SPKF202003）；黑龙江省属高等学校基本科研业务费科研项目（135309507） |
| Author Name | Affiliation | | CAO Zhijun1,2,3, CHEN Jiapeng1, REN Jian1,2,3, SONG Chunli1,2,3, LIU Jun3 | 1.College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, Heilongjiang, China；
2.Heilongjiang Engineering Research Center of Corn Staple Food Industrialization, Qiqihar
161006, Heilongjiang, China； 3.Heilongjiang Key Laboratory of Corn Deep Processing
Theory and Technology, Qiqihar 161006, Heilongjiang, China |
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| Abstract: |
| In order to improve the properties of soybean protein isolates film, the enzymatic hydrolysates of zein (ZH) was prepared by alkaline protease. The cross-linking reaction between soybean protein isolates (SPI) and ZH was catalyzed by transglutaminase (TGase), and the reaction conditions were optimized. Four modified products(0 h-SPI/ZH, 0.5 h-SPI/ZH, 1 h-SPI/ZH, and 1.5 h-SPI/ZH) were obtained by controlling the reaction time, and the properties of the film produced by the modified products were investigated. The results showed that the optimal reaction conditions were as follows: mass ratio of SPI to ZH 3.5∶ 1, substrate mass concentration 40 mg/mL, pH 7.5, TGase addition 10 U/g(based on the mass of protein), reaction time 1.5 h and reaction temperature 45 ℃. With the prolonging of reaction time, the tensile strength and opacity of the composite film prepared by the four modified products increased, while the water content, elongation at break and water vapor transmission rate decreased. Compared with SPI film, the tensile strength of the composite film prepared by 1.5 h-SPI/ZH increased by 5.5%, the water content and water vapor transmission rate decreased by 25.6% and 322%, respectively, and the opacity increased by 114.2%. In conclusion, the overall quality of the composite film prepared by TGase catalyzed cross-linking of ZH and SPI is good, providing technical support for the development of SPI composite film. |
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