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| Factors affecting the regioselectivity of lipase |
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| DOI： |
| KeyWord:lipase regioselectivity structured lipid |
| FundProject:泰山学者工程（黄凤洪）；山东省重点研发计划（医用食品）（2019YYSP027） |
| Author Name | Affiliation | | KANG Jiahuizi1,2, WANG Xiuxiu2, JIA Min2, XU Tongcheng1,2， ZONG Aizhen2,
DU Fangling2， HUANG Fenghong2， YOU Yanli1 | 1.School of Life Sciences, Yantai University,Yantai 264005, Shandong, China
2.Institute of
Agro-Food Science and Technology/Shandong Engineering Research Center for Formulated Foods for
Special Medical Purposes/Key Laboratory of Advanced and Deep Processing Technology of Agricultural
Products of Shandong Province/Key Laboratory of New Food Resource Processing of Ministry of
Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan 250100, China |
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| Abstract: |
| To develop efficient, green and low-cost lipases for the preparation of structured lipid, the factors affecting lipase regioselectivity were discussed in terms of both endogenous (source of lipase, gene sequence, spatial resistance) and exogenous (substrate spatial structure, reaction medium, immobilization conditions and reaction pH). The lipases catalytic specificity were determined by the endogenous and exogenous factors, and changing the substrate spatial structure, reaction medium, immobilization conditions and reaction pH, were often used as treatment ways to improve the catalytic performance of the lipase. Only by fully considering the effects of both internal and external factors, the regioselectivity and catalytic efficiency could be improved. The summarize is expected to develop new research directions for the development of highly selective lipase and provide enlightening research ideas for the subsequent efficient synthesis of structured lipid. |
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