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| Optimization of ultrasound-microwave assisted extraction of tea saponins from oil-tea camellia seed cake |
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| DOI： |
| KeyWord:oil-tea camellia seed cake tea saponins ultrasound-microwave assisted extraction Plackett-Burman test design response surface methodology |
| FundProject:国家重点研发计划项目（2018YFD0401000） |
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| Abstract: |
| In order to improve the extraction rate of tea saponins and expand the application of tea saponins, tea saponins was extracted by ultrasound-microwave assisted method from defatted oil-tea camellia seed cake. The extraction rate of tea saponins was taken as the index, a Plackett-Burman test design was used to screen out the test factors with significant effects based on a single-factor experiment, then the process parameters were optimized by response surface methodology. In addition, the ultrasound-microwave assisted extraction process of tea saponins was compared with ultrasound assisted process and microwave assisted extraction process. The results showed that the optimal extraction parameters of ultrasound-microwave assisted method were as follows: ethanol volume fraction 59%, solid-liquid ratio 1∶ 12, extraction solution pH 7, ultrasonic power 475 W, ultrasonic time 4 min, 2 s interval and 2 s ultrasonic work, microwave power 480 W, extraction temperature 59 ℃, microwave extraction time 8 min. Under these optimal parameters, the extraction rate and purity of tea saponins were 98.40% and 64.7%, respectively. The extraction rates of tea saponins by ultrasonic assisted method and microwave assisted method were 69.06% and 71.40%, respectively. Compared with ultrasound assisted method and microwave assisted method, ultrasound-microwave assisted method can significantly improve the extraction rate of tea saponins. |
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