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| Protection effect of Koelreuteria paniculata seed oil on non- alcoholic fatty liver disease |
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| DOI： |
| KeyWord:Koelreuteria paniculata seed oil NAFLD Lo-2 lowering lipid and protecting liver |
| FundProject:教育部“春晖计划”合作科研项目（14）；陕西省科技厅项目（2021SF-390） |
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| Abstract: |
| In order to promote the development and utilization of Koelreuteria paniculata plant resources, the protection effect of Koelreuteria paniculata seed oil against non-alcoholic fatty liver disease（NAFLD） was investigated through in vitro cellular experiments and in vivo animal experiments. In vitro experiments were conducted using free fatty acid (FFA)-induced human hepatocytes (Lo-2) to establish a NAFLD cell model to observe the effects of Koelreuteria paniculata seed oil intervention on steatosis and triglyceride (TG) content in Lo-2 cells, and to observe the effect of Koelreuteria paniculata seed oil on lipid accumulation in the NAFLD cell model. Through in vivo experiments, a mice model of NAFLD was established using high-fat diet feeding. After successful modelling, the mice were administered continuously with Koelreuteria paniculata seed oil by gavage for 7 d. Serum and liver tissues of the mice were collected to observe liver histopathology, and the levels of total cholesterol (TC), TG, glutamic transaminase (AST) and glutamic aminotransferase (ALT) were measured to investigate the hepatoprotective effects of Koelreuteria paniculata seed oil on mice with NAFLD. The results showed that NAFLD cell models were established by FFA induction of Lo-2, and after Koelreuteria paniculata seed oil intervened in the model cells for 24 h, the proliferation ability of Lo-2 cells was enhanced and they showed certain cytoprotective ability. The results of oil red O staining showed that Koelreuteria paniculata seed oil could significantly reduce the accumulation of lipids in FFA-induced Lo-2 cells and lower the content of TG, and the effect was concentration-dependent. Experiments on NAFLD mice showed that Koelreuteria paniculata seed oil could lower blood lipids, improve lipid accumulation, and protect the liver. In conclusion, from in vivo and in vitro experiments, Koelreuteria paniculata seed oil can reduce the accumulation of intracellular lipids, thus achieving a lipid-lowering effect. |
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