| In order to promote the high value utilization of high oleic acid peanut oil, cocoa butter equivalent was synthesized by enzymatic transesterification using high oleic acid peanut oil as raw material, ethyl palmitate and ethyl stearate as acyl donors in a solvent-free system. Taking the contents of target triglycerides POP,POS and SOS, stearic index and acyl migration rate as evaluation indexes, the process conditions of enzymatic synthesis of cocoa butter equivalent were optimized by response surface methodology on the basis of single factor experiment. The results showed that the optimal reaction conditions were obtained as follows: molar ratio of ethyl stearate to ethyl palmitate 1.3∶ 1, molar ratio of acyl donors to high oleic acid peanut oil 12∶ 1, enzyme loading amount 2.7%(based on the mass of substrate), reaction temperature 60 ℃ and reaction time 7 h. Under these conditions, the contents of POP, POS and SOS were 14.55%, 48.87% and 25.17% respectively, the stearic index was 0.56, and the acyl migration rate was 7.35%. The triglyceride and fatty acid composition of the product are similar to those of cocoa butter, and it can be applied as the cocoa butter equivalent. |
