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| Optimization of enzyme assisted extraction and purification of rice bran protein |
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| DOI： |
| KeyWord:rice bran protein extraction purification alkaline protease cellulase |
| FundProject: |
| Author Name | Affiliation | | HU Bo1, GAO Pan 1,2,3, MAO Yanni3,4, TIAN Jie1, ZHONG Wu1,2,3,
HU Chuanrong1,2, HE Dongping1,2,3 | 1.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
2.Key Laboratory for Deep Processing of Major Grain and Oil of Ministry of Education, Wuhan 430023,
China
3.Key Laboratory of Edible Oil Quality and Safety for State Market Regulation,
Wuhan 430012, China
4.Wuhan Institute for Food and Cosmetic
Control, Wuhan 430012, China |
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| Abstract: |
| In order to provide guidance for the deep processing and utilization of rice bran by-products, the rice bran protein was extracted using alkaline protease assisted alkaline dissolution and acid precipitation method, and further purified with cellulase. The extraction and purification process conditions were optimized by orthogonal experimental based on single factor experiment. The results showed that the optimal conditions of extraction of rice bran protein were obtained as follows:enzymatic hydrolysis temperature 50 ℃, enzymatic hydrolysis pH 10.5, enzymatic hydrolysis time 120 min, material-liquid ratio 1∶ 10, and dosage of enzyme 2.5%. Under these conditions, the extraction rate of rice bran protein was 75.2%. The optimal conditions of purification of rice bran protein were obtained as follows:enzymatic hydrolysis temperature 50 ℃, enzymatic hydrolysis pH 5.0, enzymatic hydrolysis time 60 min, material-liquid ratio 1∶ 10, and dosage of enzyme 4%. Under these conditions, the purity and extraction rate of rice bran protein were 81.6% and 72.6%, respectively. The rice bran protein with high purity and extraction rate can be obtained by this method. |
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