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| Powdered-nanoliposome loaded with DHA algal oil: effects of wall materials on storage stability, in vitro digestibility and cellular uptake |
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| DOI： |
| KeyWord:DHA nanoliposome spray drying stability digestion cellular uptake |
| FundProject:浙江省“尖兵凌雁”科技企业基金(2022C04009)；浙江省自然科学基金(LGJ20C200001) |
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| Abstract: |
| In order to develop DHA algal oil-loaded liposome powder with higher storage stability and bioavailability, the liposome suspension loaded with DHA algal oil was prepared by using DHA algal oil and soy lecithin as raw materials, and then three kinds of wall materials (maltodextrin, hydroxypropyl-β-cyclodextrin and their mixture with a mass ratio of 1∶ 1) were used to embed the liposome suspension and spray drying to obtain powdered-nanoliposome loaded with DHA algal oil. In addition, the effects of the three wall materials on the oxidative stability, in vitro digestibility and cellular uptake of the liposome powder were compared. The results showed that the optimal conditions for the preparation of DHA algal liposome suspension were as follows: mass ratio of soy lecithin to algal oil 3∶ 1, ultrasonic time 1 min, evaporation temperature 45 ℃, PBS pH 7.0, PBS concentration 0.02 mol/L, PBS dosage 10 mL (mass of soy lecithin and DHA algal oil 0.4 g). Under the optimal conditions, the encapsulation rate of DHA algal oil liposome suspension was 8801%. The particle size range of the three liposome powders was between (253.87±1.96) - (408.80±1.23) nm, and the Zeta potential was (-40.80±0.78)-(-34.87±0.25) mV. When the mixture of hydroxypropyl-β-cyclodextrin and maltodextrin was used as wall material, powdered-nanoliposome loaded with DHA algal oil had higher oxidation stability, lower water content and slower in vitro digestion release rate of fatty acids. The three liposome powder increased the expression of SLC27 A4, FABP4 and CD36 genes in Caco-2 cells, indicating that the liposome powders had good cell uptake. In conclusion, DHA algal oil nanoliposomes prepared based on soy lecithin and spray drying technology can effectively improve the oxidative stability of DHA algal oil. |
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