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| Determination of four 3-chloro-1,2-propanediol diesters in refined vegetable oil by liquid chromatography tandem mass spectrometry |
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| DOI： |
| KeyWord:3-chloro-1,2-propanediol diester refined vegetable oil non-derivatization LC-MS-MS technology |
| FundProject:南通市科技项目计划（JC2020109,MS12021027） |
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| Abstract: |
| For the accurate and rapid determination of 3-chloropropanol diesters in vegetable oil, a method for the determination of four kinds of 3-chloro-1,2-propanediol diesters,including 3-chloro-1,2-propanediol palmitate diester (3-MCPD-PP), 3-chloro-1,2-propanediol dioleate (3-MCPD-OO), 3-chloro-1,2-propanediol stearic acid diester (3-MCPD-SS) and 3-chloro-1,2-propanediol-1-palmitate-2-linoleate diester (3-MCPD-PL) in refined vegetable oil by liquid chromatography tandem mass spectrometry (LC-MS-MS) was established.The oil sample was added with deuterium isotope internal standard, then cleaned-up by NH2 solid phase extraction column and solid phase dispersion extraction clean-up kit（PSA 500 mg， C18 500 mg）. After passing 0.22 μm organic membrane, 2 μL sample solution was injected into liquid chromatography-mass spectrometry for determination.After separation by Accucore VDX column, ionization by ESI source, scanning by SRM mode and qualitative and quantitative analysis of 3-chloro-1,2-propanediol diesters by isotope internal standard method.The results showed that there was a good linear relationship when the mass concentration of four 3-chloro-1,2-propanediol diesters was in the range of 1-1 000 μg/L，and the detection limits of the four 3-chloro-1,2-propanediol diesters were 0.010 mg/kg.The recoveries and relative standard deviations in refined vegetable oil were 3-MCPD-PP 62.3%-94.0% and 5.43%-11.67%, 3-MCPD-OO 60.1%-91.7% and 3.28%-13.54%, 3-MCPD-SS 616% -92.1% and 4.73%-10.46%, 3-MCPD-PL 62.3% -92.2% and 2.69%-11.37%, respectively.The method is simple, efficient and can be used for the determination of 3-chloro-1,2-propanediol diesters in refined vegetable oils. |
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