| In order to investigate the differences in olive fruit oil quality among different varieties at the gene level and the metabolic pathways of their differential genes, forth mature fruits of two olive varieties of ‘Chenggu-32’ and ‘Koroneiki’ taken from Longnan, Gansu province were used as materials, and the differences in oil composition and physicochemical properties of olive fruits of two varieties were studied,then RNA-seq technology was used to analyze the transcriptomes to explore the key expression genes and their metabolic pathways with the differences in the quality of olive oil from different varieties. The results showed that there were obvious differences between ‘Chenggu-32’ and ‘Koroneiki’ in fatty acid composition and content, total phenol and total flavonoid contents, acid value and peroxide value, and the oil quality of ‘Koroneiki’ was superior to ‘Chenggu-32’. 10 114 differential genes were screened in the two olives. These differential genes were associated with pathways related to nucleotide synthesis, metabolism and bioregulation, mainly were rich in the metabolic pathways and secondary metabolite biosynthesis pathways. The differential expressed genes significantly up-regulated or down-regulated in the pathways of photosynthesis, carbon fixation metabolism, glutathione metabolism and cytochrome P450 metabolism were important to the formation of differences in olive oil. |
