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| Extraction of Moringa oleifera seed oil and purification technology of nervonic acid |
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| DOI： |
| KeyWord:Moringa oleifera seed oil nervonic acid low temperature crystallization method response surface methodology |
| FundProject:云南省重大科技专项（202102AE090027） |
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| Abstract: |
| In order to promote the development and utilization of nervonic acid in Moringa oleifera seed oil, with Moringa oleifera seed as raw material, Moringa oleifera seed oil was extracted by low temperature pressing, supercritical CO2 extraction and Soxhlet extraction to select suitable oil extraction method. Then, mixed fatty acids were prepared using Moringa oleifera seed oil as raw material, and the nervonic acid in Moringa oleifera seed oil was purified by low temperature crystallization method. The purification process of nervonic acid was optimized by single factor experiment combined with response surface methodology. The results showed that the content of nervonic acid in crude Moringa oleifera seed oil extracted by low temperature pressing method was higher, reaching 0.88%.The optimal purification process of nervonic acid from Moringa oleifera seed oil by low temperature crystallization method was obtained as follows: solid-liquid ratio(ratio of mixed fatty acid mass to crystallization solvent volume) 1∶ 4.2, crystallization temperature -21 ℃ and crystallization time 2.3 h. Under these conditions, the nervonic acid content in the product was (5.36±0.01)%, which was 5.1 times higher than that in crude Moringa oleifera seed oil. By extracting Moringa oleifera seed oil through low temperature pressing method and purifying nervonic acid through low temperature crystallization method, products with high levels of nervonic acid can be obtained. |
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