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| Nitrogen regulatory strategies for promoting efficient enrichment of energy storage compounds in Tribonema sp. |
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| DOI： |
| KeyWord:Tribonema sp. nitrogen form two-step method energy storage compound "low nitrogen to high nitrogen" culture |
| FundProject:湖北省自然科学基金 (2021CFB224);江苏省自然科学基金（BK20200734）;武汉轻工大学科研项目资助（2021Y06）；湖北省高校优秀中青年科技创新团队(T2020012);湖北省重点研发项目(2020BBA043) |
| Author Name | Affiliation | | GUO Yuhao1, LI Yuanhong2, CAO Yan2, WANG Feifei1,2, ZHU Zhenzhou1,2 | 1.National R&D Center for Se-rich Agricultural Products Processing, School of Food Science and
Engineering, Wuhan Polytechnic University, Wuhan 430023, China
2.School of Modern Industry for Selenium Science and Engineering, Wuhan
Polytechnic University,Wuhan 430048, China |
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| Abstract: |
| In order to promote efficient enrichment of energy storage compounds in Tribonema sp. , the effects of different nitrogen forms (NO-3, Urea and NH+4) and initial nitrogen concentration (1, 3, 6, 9, 18 mmol/L) on the growth, carbohydrate and lipid accumulation of Tribonema sp. were investigated. Then a two-step culture strategy from low nitrogen (initial nitrogen concentration 3 mmol/L) to high nitrogen (initial nitrogen concentration 18 mmol/L) based on nitrogen regulation was established to promote the simultaneous and efficient accumulation of biomass and energy storage compounds in the alga, and the culture time of the two-step culture strategy was optimized. The results indicated that the highest biomass concentration (3.69 g/L) and total lipid content (57.3%) were obtained at 3 mmol/L and 18 mmol/L of NO-3 in Tribonema sp. , respectively, compared with Urea and NH+4. In the two-step culture strategy of "low nitrogen to high nitrogen", 6 d of low nitrogen culture to 12 d of high nitrogen culture could significantly increase the accumulation of energy storage compounds in Tribonema sp. , and the highest energy storage compounds yield was 274.26 mg/(L·d), of which the biomass and yields of total lipid and carbohydrate were 6.37 g/L, 152.92 mg/(L·d), 121.35 mg/(L·d), respectively.The two-step nitrogen regulation based on "low nitrogen to high nitrogen" is a promising culture model that can significantly increase biomass and carbohydrate yield while ensuring comparable total lipid yield. |
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