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| Optimization of fermentation conditions of Schizochytrium sp. mutant with high DHA yield |
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| DOI： |
| KeyWord:DHA Schistochytrum sp. mutant strain optimization of fermentation conditions |
| FundProject:2023武汉轻工大学校立科研项目（01500001） |
| Author Name | Affiliation | | DING Yunpeng1, LYU Xiaoyi1, LI Hengbin1, YANG Yong2,3, ZHANG Han1,
HU Bo1, GAO Pan1,3,4, HU Chuanrong1,3, HE Dongping1,3,4, ZHONG Wu1,3,4 | 1.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023,China
2.Wuhan Institute for Food and Cosmetic Control, Wuhan 430012,China
3.Key Laboratory of
Edible Oil Quality and Safety for State Market Regulation, Wuhan 430023,China
4.Key Laboratory for Deep Processing of Major Grain and Oil of Ministry of
Education in China, Wuhan 430023,China |
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| Abstract: |
| In order to further increase the DHA yield of Schizochytrium sp. mutant, the Schizochytrium sp. mutant 1.6-7-1 with high yield DHA obtained by 60Co-γ radiation mutagenesis was used as research object, and the fermentation medium of DHA production by the Schizochytrium sp. mutant was further optimized by Plackett-Burman experiment, steepest ascent experiment and response surface methodology. In addition, the effects of different dissolved oxygen levels on the metabolism of the mutant strain were investigated through fermentation cultivation in fermentation tank. The results showed that the dosages of glucose, sodium glutamate and NaCl had significant effects on the DHA production of the mutant strain, and their optimum dosages were glucose 125.46 g/L, sodium glutamate 12.44 g/L and NaCl 4 g/L. Under the optimum conditions, the DHA production could reach 6.01 g/L, which increased by 49.88% compared with the original strain.Under high dissolved oxygen conditions, the mutant strain exhibited higher biomass accumulation but lower oil and DHA production, which possibly caused by the preferential use of nutrients for the own growth and reproduction of cells. Conversely, low dissolved oxygen inhibited energy metabolism and slowed down cell proliferation. Therefore, optimizing fermentation conditions can increase the DHA yield of the mutant strain. |
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