|
| Effects of media, light intensity and initial nitrogen concentration on the growth and bioactive metabolites accumulation of |
| |
| DOI： |
| KeyWord:Klebsormidium sp. media light intensity nitrogen concentration lipid fatty acid lutein |
| FundProject:广东省基础与应用基础研究基金项目（2020A1515110802）;国家自然科学基金（32002412） |
|
| Hits: 2483 |
| Download times: 3198 |
| Abstract: |
| To efficient production of important metabolites such as oil, linoleic acid and lutein by Klebsormidium sp. JNU41, the culture conditions were optimized and the accumulation of metabolites in Klebsormidium sp. JNU41 was evaluated. The growth curve, biochemical composition, total lipid yield, fatty acid composition and lutein content of Klebsormidium sp. JNU41 were studied using modified BG-11 (mBG-11) and BBM (mBBM) as the media, and three sets of light intensities 〔unilateral 100 μmol/(m2·s), unilateral 300 μmol/(m2·s) and bilateral 300 μmol/(m2·s)〕 and six initial nitrogen concentrations (3, 6, 9, 12, 15, 18 mmol/L) were used for culture. The results showed that under the conditions of mBBM media, bilateral light intensity 300 μmol/(m2·s) and initial nitrogen concentration 6 mmol/L, the biomass (8.38g/L) and total lipid yield 〔281.71 mg/(L·d)〕 reached a maximum value. Under the conditions of mBG-11 media, bilateral light intensity 300 μmol/(m2·s) and initial nitrogen concentration 3 mmol/L, the highest total lipid and total fatty acid contents reached 55.12% and 47.10%, respectively. The main components of fatty acids were palmitic acid, oleic acid, linoleic acid and linolenic acid, and linoleic acid content was the highest with the highest relative content of 65.87%. In addition, Klebsormidium sp. JNU41 also accumulated a certain amount of lutein, up to 1.64 mg/g. Therefore, Klebsormidium sp. JNU41 is an algal strain that can be used to produce high value-added products. |
| View full text View/Add Comment Download reader |
| Close |
|
|
|
