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| Construction of bacterial carrier by modified activated carbon and its adsorption of AFB1 from maize |
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| DOI： |
| KeyWord:magnetic activated carbon Bacillus spp. bacterial carrier AFB1 biological removal |
| FundProject:甘肃省重点研发计划——农业类(20YF8NA041) |
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| Abstract: |
| Aiming to provide technical support for biological treatment of aflatoxin-contaminated grain feed, magnetic activated carbon (CSH) was prepared through modification from activated carbon(SH), and the strain of Bacillus spp. WTX1, which was capable of efficiently degrading aflatoxin B1 (AFB1), was selected to co-culture with the CSH carrier to construct the magnetic bacterial carrier (FCSH) for the removal of AFB1 from contaminated maize. BET specific surface area, nitroqen adsorption-desorption isotherms and isotherm adsorption curves for AFB1 of CSH were analyzed, and the effect of FCSH construction conditions and FCSH adsorption conditions on the effect of AFB1 removal were investigated. Fourier transform infrared spectrum (FTIR) and scanning electron microscope were used to characterize FCSH before and after adsorption of AFB1. The results showed that CSH had a macroporous structure, and its isothermal adsorption of AFB1 conformed to the Freundlich equation. The removal rate of AFB1 by FCSH could reach 97.45% under the conditions of immobilization time 16 h, immobilization and adsorption speed 160 r/min, immobilization and adsorption temperature 35 ℃, adsorption pH 8, and shaking time 48 h. The adsorption of FCSH on AFB1 was confirmed by FTIR and scanning electron microscope, and the adsorption effect of FCSH on AFB1 was stronger than that of CSH.In conclusion, the prepared FCSH can effectively remove AFB1 from maize. |
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