Preparation of blend oil with balanced nutrition and its nutritional components determination
  
DOI:
KeyWord:medium-and long-chain triacylglycerols  α-linolenic acid  enzymatic synthesis  oxidative stability
FundProject:财政部和农业农村部国家现代农业产业技术体系资助(CARS-18-ZJ0503);佛山市财政专项资金——高水平广东省农业科技示范市建设资金市院合作项目;广东省农业科学院地方分院和专家工作站工作经费项目(2023支撑01)
Author NameAffiliation
CHEN Funi1,2, LIU Chen2, WANG Weifei2, ZHONG Saiyi1, WANG Siyuan2, MU Lixia2, LIAO Sentai2, ZOU Yuxiao2 1.College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, Guangdong, China 2. Guangdong key Laboratory of Agricultural Products Processing, Key Laboratory of Functional Food, Ministry of Agriculture and Rural Affairs, Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China 
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Abstract:
      In order to provide reference for the application of medium-and long-chain fatty acid triglycerides (MLCT) products enriched with polyunsaturated fatty acids (PUFA), lipase Lipozyme TL IM was employed to catalyze the transesterification of refined silkworm pupa oil (SPO) and tricaprylin (TRI) to synthesize MLCT rich in α-linolenic acid (ALA) (ALA-MLCT). The impact of reaction time on the synthesis of ALA-MLCT was studied, and the triglycerides composition, quality index, oxidative stability, emulsion performance, and in vitro digestion characteristics of the ALA-MLCT were analyzed. The results showed that Lipozyme TL IM had high catalytic efficiency, and the conversion rate of TRI reached more than 98% after 10 h of reaction. The content of MLCT containing ALA in ALA-MLCT synthesized by transesterification reaction for 12 h reached 40.41%, and it was mainly composed of LnCaCa (10.95% ), PLnCa (769%) and OLnCa (6.21%). The quality indexes of ALA-MLCT complied with the standards of first-class edible oil in the medium- and long- chain fatty acid edible oil standard draft 2022. Compared with SPO and the physical mixture of transesterification reaction substrate (SPO+TRI, in a 1∶ 4 mass ratio), ALA-MLCT exhibited significantly improved oxidation stability, and its emulsion had better physical stability, with smaller and more evenly distributed particles size. In vitro simulated digestion experiments revealed that ALA-MLCT achieved a faster digestive balance compared with SPO+TRI, but the final digestibility of both could exceed 90%. In conclusion, the synthesized ALA-MLCT can help to improve the bioaccessibility of ALA and is expected to be used as functional edible oil.
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