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| Effect of thioesterase gene expression of Corynebacterium glutamicum on fatty acid synthesis |
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| DOI： |
| KeyWord:thioesterase heterologous expression homologous expression fatty acid |
| FundProject:国家自然科学基金（31860439） |
| Author Name | Affiliation | | WANG Jia1,2, LIU Jin1, SUN Ziyu1, Mandlaa1, CHEN Zhongjun1 | 1.College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010000, China
2.Inner Mongolia Fengzhen Inspection and Testing Center, Fengzhen 012100,
Inner Mongolia, China |
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| Abstract: |
| In order to lay the foundation for further construction of fatty acid high-yield strains, the effects of the genes for thioesterases (NCgl2365 and NCgl0090) in Corynebacterium glutamicum ATCC13032 on fatty acid synthesis were determined by heterologous and homologous expression. The results showed that overexpression of thioesterases affected both the type and content of fatty acids in Escherichia coli and Corynebacterium glutamicum. Gene NCgl2365 increased fatty acid production by 3786% after overexpression in Escherichia coli and promoted the production of saturated fatty acids C16∶ 0, C18∶ 0, and unsaturated fatty acid C17∶ 1 and C20∶ 3; fatty acid production increased by 3343% after homologous expression in Corynebacterium glutamicum, and the relative content of C17∶ 0 increased by 51.82%, whereas the relative contents of C6∶ 0, C8∶ 0, C12∶ 0, C17∶ 1, C18∶ 0, C18∶ 1 and C18∶ 3 decreased. Fatty acid production increased by 58.15% after overexpression of gene NCgl0090 in Escherichia coli, which contributed to the production of saturated fatty acids C14∶ 0,C16∶ 0, C18∶ 0, and unsaturated fatty acid C17∶ 1; fatty acid production increased by 59.12% after homologous expression in Corynebacterium glutamicum, with 40.18% increase in the relative content of C16 ∶ 0, the synthesis of eight new fatty acids, C10∶ 0,C14∶ 0, C14∶ 1, C15∶ 0, C16∶ 1, C18∶ 2, C20∶ 1 and C21∶ 0 was promoted. In conclusion, the genes NCgl2365 and NCgl0090 exerted important effects on fatty acid metabolism in the host bacteriophage. |
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