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| Effects of cultivation conditions on growth and synthesis of linoleic acid of filamentous microalgae Klebsormidium sp. |
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| DOI： |
| KeyWord:Klebsormidium sp. cultivation condition oil linoleic acid pH |
| FundProject:国家重点研发计划项目（2021YFA0909704）；国家自然科学青年科学基金项目（31600310） |
| Author Name | Affiliation | | ZHAO Danyue1, LI Lu2, CHEN Lin2, LIU Tianzhong2 | (1.School of Food Science and Engineering, Ocean University of China, Qingdao 266003, Shandong, China
2.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences,
Qingdao 266101, Shandong, China) |
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| Abstract: |
| In order to develop new resources for the production of linoleic acid, with a filamentous microalgae Klebsormidium sp. isolated as object, the effect of nitrogen concentration, phosphorus concentration in medium, and light intensity, temperature and environmental pH on the growth, nitrogen consumption, oil content and fatty acid composition of Klebsormidium sp. were investigated. The results showed that nitrogen concentration in the medium did not affect the growth of Klebsormidium sp. , but high nitrogen concentration was detrimental to the synthesis of oil and unsaturated fatty acids. Low phosphorus concentration in the medium inhibited cell growth, while high phosphorus concentration had a toxic effect on cells. Appropriately increasing light intensity was beneficial to cell growth, but had little effect on oil accumulation. More unsaturated fatty acids such as linoleic acid was synthesized under lower light intensity cultivation. Temperature had little effect on the growth and oil accumulation of Klebsormidium sp. , but low temperature was beneficial to the synthesis of linoleic acid. The growth, oil accumulation and linoleic acid content of Klebsormidium sp. were significantly affected by the environmental pH. Cells grew the fastest and had higher oil content in weakly acidic-neutral environments with pH 6.0-7.5. Under the conditions of nitrogen concentration 3-6 mmol/L, phosphorus concentration 0.115-0.230 mmol/L, light intensity 100-300 μmol/(m2·s), temperature 15-30 ℃, environmental pH 7.5, the maximum oil content reached about 42%, of which linoleic acid accounted for 65%-75%. In summary, filamentous microalgae Klebsormidium sp. is a promising new resource for linoleic acid production. |
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