| In order to provide reference for the improvement of purification process of soluble soybean polysaccharide（SSPS）and the comprehensive utilization of soybean dregs, the SSPS crude products were obtained from dried soybean dregs by dynamic extraction and purified by enzymatic hydrolysis, activated carbon adsorption and alcohol precipitation. The purification process was optimized and the effects of purification on the basic component, physicochemical property, volatile components, structure and properties of SSPS were investigated. The results showed that the optimal conditions for enzymatic hydrolysis were 1.0% papain addition and 2 h enzymatic hydrolysis at pH 6 and 60 ℃; the optimal conditions for activated carbon adsorption were 1.0% activated carbon addition and adsorption for 30 min at pH 5 and 65 ℃. After purification, the protein and ash contents of SSPS decreased from 3.60% and 5.13% to 2.19% and 4.73%, respectively. The transparency of the purified SSPS increased by 71.94%, the viscosity decreased, the number of volatile components decreased by 18, while the molecular mass did not change significantly. The molecular structure of purified SSPS was similar to that of commercial SSPS, but there were some differences in crystallinity. The foaming and emulsifying properties of purified SSPS reached 213.70% and 0.76, respectively, and the foam stability and emulsifying stability were better than those of commercial SSPS and acacia. In summary, the property of SSPS crude products extracted by dynamic method was improved after purification. |
