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Effect of ultrasound treatment on structure and physicochemical properties of Eucommia ulmoides seed kernel protein |
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DOI: |
KeyWord:Eucommia ulmoides seed kernel protein ultrasound treatment microstructure physicochemical property |
FundProject:河南省重大科技专项(231100110400) |
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Abstract: |
To provide reference for the development and application of Eucommia ulmoides seed kernel protein, the Eucommia ulmoides seed kernel meal was used as material, the protein was extracted with alkali solution and acid precipitation, and Eucommia ulmoides seed kernel protein was treated by ultrasound (500 W, 35 kHz) for different time (0, 10, 20, 30 min and 40 min). The effects of ultrasound treatment on Eucommia ulmoides seed kernel protein′s microstructure and physicochemical properties (particle size, Zeta-potential, fluorescence spectrum, solubility, water holding capacity, oil holding capacity, foaming capacity, foam stability, emulsifying capacity, emulsifying stability) were studied.The results showed that ultrasound treatment could change microstructure, particle size, Zeta-potential and fluorescence intensity of Eucommia ulmoides seed kernel protein. When the ultrasound treatment time was 20 min, the solubility of Eucommia ulmoides seed kernel protein reached 75.35%. When the ultrasound treatment time was 10 min, the emulsifying capacity of Eucommia ulmoides seed kernel protein was the highest, reaching 72.12 m2/g. When the ultrasound treatment time was 40 min, the water holding capacity, oil holding capacity,foaming capacity and foam stability of Eucommia ulmoides seed kernel protein were the largest, reaching 4.32 g/g, 3.46 g/g, 23.12% and 21.12%, respectively. In summary, ultrasound treatment can improve the functional characteristics of Eucommia ulmoides seed kernel protein,such as water holding capacity, oil holding capacity, foaming capacity and emulsifying capacity. |
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