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| Optimization of enzymatic hydrolysis preparation process of Moringa oleifera seed sobering peptide |
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| DOI： |
| KeyWord:Moringa oleifera seed sobering peptide hydrolysis degree ADH activation rate |
| FundProject:国家自然科学基金地区项目（31960462）;云南省农业联合专项（202101BD070001-013）;云南省科协第三届青年科技人才托举工程项目 |
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| Abstract: |
| To promote the development and utilization of Moringa oleifera seed sobering peptide, Moringa oleifera seed protein powder was used as raw material, and the hydrolysis degree of protein and the alcohol dehydrogenase (ADH) activation rate of sobering peptide were used as indicators, single factor experiment and response surface methodology were used to optimize the preparation process of Moringa oleifera seed sobering peptide. The results showed that the optimum enzymatic hydrolysis conditions for the preparation of Moringa oleifera seed sobering peptide were as follows: solid-liquid ratio 1∶ 20, addition amount of alkaline protease 5.0%, enzymatic hydrolysis pH 9.0, enzymatic hydrolysis temperature 63 ℃, and enzymatic hydrolysis time 142 min. Under these conditions, the hydrolysis degree of Moringa oleifera seed protein was 12.23%, and the ADH activation rate of Moringa oleifera seed sobering peptide was 22.16%. In conclusion, the preparation of sobering peptide from Moringa oleifera seed has a good prospect for development. |
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