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| Research progress on enzymatic preparation of human milk fat substitutes |
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| DOI： |
| KeyWord:OPL OPO human milk fat substitutes enzymatic preparation |
| FundProject:国家自然科学基金项目(32272271,31972038)；国家重点研发计划项目(2021YFD2100303)；湖北省自然科学基金(2021CFB209)；湖州市南太湖精英计划领军型创新团队项目 |
| Author Name | Affiliation | | CHEN Hangfei1,2, ZHANG Yufei2,3, LI Yali2, ZHENG Mingming1,2,3, WANG Jun1 | 1.School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212000, Jiangsu,
China
2.Oil Crops Research Institute, Chinese Academy of Agricultural Sciences/Hubei Key
Laboratory of Lipid Chemistry and Nutrition, Wuhan 430062, China
3.Huzhou
Linghu Xinwang Chemical Co. , Ltd. , Huzhou 313018, Zhejiang, China |
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| Abstract: |
| Human milk fat substitutes are a kind of structural lipids that simulate the composition of breast milk fat. In order to promote green and efficient production of human milk fat substitutes, the triglyceride composition of breast milk fat was introduced, and the enzymatic preparation technology and practical application of human milk fat substitutes were analyzed, summarized and discussed, and the future research direction was prospected. The content of 1-oleoyl-2-palmitoyl-3-linoleoyl glycerol (OPL) was the highest in Chinese breast milk fat, followed by 1,3-dioleoyl-2-palmitoyl glycerol (OPO). The enzymatic preparation of human milk fat substitutes includes ester-esterification, one-step acid hydrolysis, alcohololysis-esterification two-step, two-step acid hydrolysis, transesterification-acid hydrolysis two-step, etc. Improving the catalytic activity and location selectivity of enzymes and inhibiting acyl transfer are the future research directions of enzymatic preparation of human milk fat substitutes. |
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