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| 奇数链脂肪酸抑制脂多糖诱导的Caco-2
细胞炎症反应研究 |
| Protective effect of odd-chain fatty acids on lipopolysaccharide- induced inflammation in Caco-2 cells |
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| DOI： |
| 中文关键词: 奇数链脂肪酸；C15∶ 0；C17∶ 0 肠道炎症；肠道健康 |
| 英文关键词:OCFA C15∶ 0 C17∶ 0 intestinal inflammation intestinal health |
| 基金项目: |
| Author Name | Affiliation | | YAN Yumeng1, ZHANG Ying1, MA Qiang2, ZHANG Xingfang3,
YAN Yuanyuan1, LI Xiaoguang1, WANG Hui1 | 1.School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
2.Shanghai Pulmonary Hospital Tongji University, Shanghai 200433, China
3. School of Renji Clinical
Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China |
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| 中文摘要: |
| 为明确奇数链脂肪酸（OCFA）与人体健康的关系，采用气相色谱-质谱法（GC-MS）检测分析健康人群和结肠癌患者血清中OCFA的分布特征差异，并采用脂多糖（LPS）诱导的Caco-2细胞炎症模型探究OCFA对炎症的抑制效应，包括对细胞活性、促炎因子基因表达以及脂肪酸组成的影响。结果表明：健康人群中的OCFA（C15∶ 0和C17∶ 0）含量显著高于结肠癌人群；男性以及高龄（59岁以上）结肠癌人群中C15∶ 0和C17∶ 0水平偏低；C15∶ 0和C17∶ 0对细胞活力无影响，C15∶ 0能够显著降低LPS刺激后Caco-2细胞中促炎因子TNF-α、IL-8和iNOS的表达，C17∶ 0能够显著降低促炎因子IL-6、TNF-α、IL-8和iNOS的表达，两种OCFA均能够起到类似于n-3 PUFA缓解炎症刺激的作用；两种OCFA（C15∶ 0和C17∶ 0）能够在肠道细胞Caco-2中吸收并代谢成C17∶ 0、C17∶ 1。综上，OCFA能够调节肠道炎症因子的表达进而抑制肠上皮细胞炎症反应，对肠道健康有潜在的保护作用。 |
| 英文摘要: |
| In order to explore the health effects of odd-chain fatty acids (OCFA), serum OCFA levels in the healthy individuals and colon cancer patients were measured by gas chromatography-mass spectrometry (GC-MS) to investigate the differences in the distribution characteristics, and the protective effects of OCFA on inflammation by using the lipopolysaccharide (LPS)-induced Caco-2 cell inflammation model were studied, including the changes in cell activity, pro-inflammatory factor gene expression and fatty acid composition. The results showed that the levels of two main OCFA (C15∶ 0 and C17∶ 0) in healthy individuals were significantly higher than that in colon cancer patients.The levels of C15∶ 0 and C17∶ 0 in male patients and patients over the age of 59 were significantly lower. C15∶ 0 and C17∶ 0 had no effect on cell activity. C15∶ 0 could significantly inhibit the expression of pro-inflammatory factors TNF-α, IL-8 and iNOS in Caco-2 cells with LPS stimulation, C17∶ 0 could significantly reduce the expression of IL-6, TNF-α, IL-8 and iNOS. Two main OCFA could play a similar role in alleviating inflammatory stimulation as n-3 PUFA. Two main OCFA could be easily incorporated by Caco-2 cells into C17∶ 0 and C17∶ 1 in Caco-2. In conclusion, OCFA may have a potential preventive effect on inflammation and intestinal health by regulating the expression of pro-inflammatory cytokines. |
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