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| Protective effect of odd-chain fatty acids on lipopolysaccharide- induced inflammation in Caco-2 cells |
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| DOI： |
| KeyWord:OCFA C15∶ 0 C17∶ 0 intestinal inflammation intestinal health |
| FundProject: |
| Author Name | Affiliation | | YAN Yumeng1, ZHANG Ying1, MA Qiang2, ZHANG Xingfang3,
YAN Yuanyuan1, LI Xiaoguang1, WANG Hui1 | 1.School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
2.Shanghai Pulmonary Hospital Tongji University, Shanghai 200433, China
3. School of Renji Clinical
Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China |
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| Abstract: |
| In order to explore the health effects of odd-chain fatty acids (OCFA), serum OCFA levels in the healthy individuals and colon cancer patients were measured by gas chromatography-mass spectrometry (GC-MS) to investigate the differences in the distribution characteristics, and the protective effects of OCFA on inflammation by using the lipopolysaccharide (LPS)-induced Caco-2 cell inflammation model were studied, including the changes in cell activity, pro-inflammatory factor gene expression and fatty acid composition. The results showed that the levels of two main OCFA (C15∶ 0 and C17∶ 0) in healthy individuals were significantly higher than that in colon cancer patients.The levels of C15∶ 0 and C17∶ 0 in male patients and patients over the age of 59 were significantly lower. C15∶ 0 and C17∶ 0 had no effect on cell activity. C15∶ 0 could significantly inhibit the expression of pro-inflammatory factors TNF-α, IL-8 and iNOS in Caco-2 cells with LPS stimulation, C17∶ 0 could significantly reduce the expression of IL-6, TNF-α, IL-8 and iNOS. Two main OCFA could play a similar role in alleviating inflammatory stimulation as n-3 PUFA. Two main OCFA could be easily incorporated by Caco-2 cells into C17∶ 0 and C17∶ 1 in Caco-2. In conclusion, OCFA may have a potential preventive effect on inflammation and intestinal health by regulating the expression of pro-inflammatory cytokines. |
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