Analysis of pigment change of cold-pressed fresh green Huajiao (Zanthoxylum schinifolium Sieb. et Zucc) oil by UPLC-DAD-HRMS
  
DOI:
KeyWord:green Huajiao  ultra-high performance liquid chromatography-diode array detector-high resolution mass spectrometry (UPLC-DAD-HRMS)  pigment  chlorophyll  carotenoid  chromaticity value
FundProject:重庆市人民政府与中国农业科学院科技创新战略合作项目(2022164);西南大学创新研究2035先导计划重点项目 (SWU-XDZD22007);西南大学大学生创新创业训练计划项目(S202210635250,X202210635487);重庆市基础研究与前沿探索项目(cstc2018jcyjAX0590);重庆市市场监督管理局科研资助项目(CQSJKJ2020023)
Author NameAffiliation
LIU Ruoxi1,2, XIAO Yuxue1,2, FENG Guiqiang1,2, ZHANG Kangzhi1,2, ZHOU Chunjie3, CAI Tian4,5, CHEN Kewei1,2,5,6,7 1.School of Food Science, Southwest University, Chongqing 400715, China
2.National Experimental Teaching Demonstration Center of Food Science and Engineering, Southwest University, Chongqing 400715, China
3.Chongqing Institute of Food and Drug Inspection, Chongqing 401121, China
4.School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China
 
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Abstract:
      Aiming to contribute to the industrial production, storage and quality control of cold-pressed fresh green Huajiao oil, the change of pigment in cold-pressed fresh green Huajiao oil was analyzed by ultra-high performance liquid chromatography-diode array detector-high resolution mass spectrometry (UPLC-DAD-HRMS), and the effects of light, oxygen, temperature on the pigment content and color(L*, a*, b*) of fresh green Huajiao oil in storage were determined. The results showed that 32 chlorophylls and 10 carotenoids were identified from the cold-pressed fresh green Huajiao oil. The main chlorophylls were chlorophyll a, C132-hydroxy chlorophyll a, chlorophyll b and pheophytin a; the main carotenoids were lutein and β-carotene. The total chlorophyll content was 9.44 mg/kg, and the total carotenoid content was 4.95 mg/kg. During storage, the main factor affecting the degradation of pigment in fresh green Huajiao oil was light, followed by temperature, and oxygen had little effect. In addition, the color index a* can be used as an index for pigment content and color control.
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