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| Screening of lipase-producing strains based on the degradation of highly oily wastewater and optimization of fermentation process |
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| DOI： |
| KeyWord:highly oily wastewater lipase Bacillus amyloliquefaciens Acinetobacter lwoffii oil-degrading bacteria enzymatic property response surface methodology |
| FundProject:贵州大学国家级大学生创新创业训练计划项目（202110657022）；国家自然科学基金地区项目（32260580）；贵州省科技厅基础研究一般项目（黔科合基础-ZK\[2021\]一般180）；中国轻工业浓香型白酒固态发酵重点实验室开放基金项目（2019JJ015） |
| Author Name | Affiliation | | ZHANG Yu1,2, WANG Qun1,2, YANG Xuehua1,2,
LIU Yelan1,2, ZENG Xiangyong1,2 | (1.College of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China
2.Guizhou Provincial Key Laboratory of Fermentation and Biopharmacy, Guizhou University,
Guiyang 550025, China) |
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| Abstract: |
| In order to screen high lipase-producing strains and explore the ability to degrade highly oily wastewater, the traditional microbial screening method was used to select the strains from the kitchen wastewater, and the oil degradation rate of highly oily wastewater (oil content 20 g/L) and the lipase activity of the selected strains were studied. Strains with higher lipase production activity were identified. Furthermore, single factor experiment and response surface methodology were employed to optimize the fermentation conditions for lipase production of the identified strains. The results indicated that three bacterial strains and two yeast strains were screened from kitchen wastewater, two bacteria grew and metabolized rapidly in highly oily wastewater, and the oil degradation rate and lipase activity were higher, which were identified as Bacillus amyloliquefaciens （Bi）and Acinetobacter lwoffii（Bu）, respectively. The optimal fermentation conditions for lipase production by Bi were pH 6.69, NaCl content 3.01 g/L, peptone content 6.01 g/L, and lipase activity was (222.83±0.91) U/mL, while that of Bu were pH 5.07, NaCl content 6.10 g/L, peptone content 9.38 g/L, and the lipase activity was (231.50±2.28) U/mL. The lipase activity of Bu after optimization even higher than that of Bi , which had a greater initial lipase activity. In conclusion, these two bacterial strains show a high biodegradation performance for highly oily wastewater, and they have a wide application prospect. |
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