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| Extraction and fatty acid composition analysis of Eisenia foetida oil |
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| DOI： |
| KeyWord:earthworm oil aqueous enzymatic method extraction process fatty acid composition |
| FundProject:校企合作项目“龙络康地龙中脂肪酸的提取分析研究”；2022年中医药重点学科建设项目“中医预防医学” |
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| Abstract: |
| In order to promote the development and utilization of by-products from earthworm protein powder processing, Eisenia fetida residue was used as the research object to extract earthworm oil using ultrasonic-assisted aqueous enzymatic-acid hydrolysis method. The effects of hydrolase type, enzymatic hydrolysis time and enzyme dosage on the yield of earthworm oil were studied, and the optimal enzymatic hydrolysis conditions were determined by orthogonal experiments. The physicochemical indexes and fatty acid composition of the earthworm oil were analyzed. The results showed that the optimal enzymatic hydrolysis conditions of earthworm oil were obtained as follows: with pepsin as the enzyme (optimal temperature 50 ℃, pH 2.0), enzymatic hydrolysis time 2.0 h and enzyme dosage 4%. Under these conditions, the yield of earthworm oil was 11.45%. The extracted crude earthworm oil was light brown, slightly cloudy, slightly earthy smell of earthworms, slightly fatty rancid taste. The earthworm oil consisted of 24 fatty acids, with high saturated fatty acid content, accounting for 82.381% of total fatty acid, mainly stearic acid and lauric acid; monounsaturated fatty acid accounted for 8.565% of total fatty acid, mainly oleic acid and palmitoleic acid; polyunsaturated fatty acid accounted for 8.995% of total fatty acid, mainly linoleic acid. In addition, the odd-chain fatty acids content in earthworm oil was 13.867%. The ultrasonic-assisted aqueous enzymatic-acid hydrolysis method has a certain effect on the extraction of oil from earthworm residue, and has practical application potential, and the obtained earthworm oil is rich in odd-chain fatty acids and has high nutritional value. |
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