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| Graded extraction and antioxidant activity of crude polysaccharides from Moringa oleifera seeds |
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| DOI： |
| KeyWord:Moringa oleifera seed polysaccharide grading alcohol precipitation antioxidant activity |
| FundProject:国家重点研发计划“中医药现代化研究”（2017YFC1702506） |
| Author Name | Affiliation | | LI Lingling1, XU Yanran1, NIU Yanfei1,2, JIN Chunxiu1,
HU Linghua1, WU Chunhua1 | (1.School of Materials and Chemical Engineering, Southwest Forestry University,
Kunming 650224, China
2.Yunnan Instutite of Materia
Medica, Kunming 650111, China) |
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| Abstract: |
| In order to better develop and utilise Moringa oleifera seeds, the differences in the composition of key monosaccharides as well as antioxidant activity of crude polysaccharides (P20, P40, P60, P80) from Moringa oleifera seeds obtained by graded alcohol precipitation with different volume fraction of ethanol (20%, 40%, 60%, 80%) were investigated, and the structure of purified polysaccharides of Moringa oleifera seeds was characterized using ultraviolet-visible spectroscopy (UV-Vis) and Fourier transform-infrared spectroscopy (FT-IR). The results showed that the main components of the four crude polysaccharides were rhamnose (Rha), galactose (Gal) and glucose (Glc), among which the crude polysaccharide P80 had the highest content of total monosaccharides (84.09 μg/mg); among the four crude polysaccharides of Moringa oleifera seeds, P20 (1 mg/mL) had the highest scavenging rate of DPPH radicals and superoxide anion radicals, with 50.24% and 34.54%, respectively, P40 (1 mg/mL) had the highest scavenging rate of hydroxyl radicals（76.92%）, and P80 had the strongest total reducing power; it was known that different volume fraction of ethanol were able to carry out graded alcohol precipitation of crude polysaccharides from Moringa oleifera seeds by UV-Vis and FT-IR. In summary, crude polysaccharides extracted with different volume fraction of ethanol have different antioxidant activities and need to be extracted according to specific applications. |
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