Metabolomics analysis of kernels of Camellia oleifera fruit treated with different methods after harvest
  
DOI:10.19902/j.cnki.zgyz.1003-7969.230133
KeyWord:Camellia oleifera fruit  kernel  postharvest treatment  metabolomics
FundProject:湖南省教育厅重点项目(22A0714);湖南省“十四五”应用特色学科(林学)(湘教通〔2022〕351);湖南应用技术学院高层次人才启动项目(2023HYBS01);常德市经济林生态过程调控与高值化利用工程技术研究中心;贵州省科技厅平台项目(黔科合平台人才〔2020〕2003)
Author NameAffiliation
LONG Xueyan1, YAN Daoliang1, GUO Chunxi2, LI Wanchun1, HU Yuling3, ZHENG Bingsong1 (1.Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Function, Zhejiang A & F University, Hangzhou 311300, China
2.Key Laboratory of Biodiversity Protection and Utilization in Fanjingshan Area of Guizhou Province
, Tongren University, Tongren 554300, Guizhou, China 3.College of Agriculture and Forestry Science and TechnologyHunan Applied Technology University Changde 415000, Hunan, China) 
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Abstract:
      In order to find the optimal postharvest treatment of Camellia oleifera fruit, and lay the foundation for subsequent research, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to determine the metabolites of the kernels of different postharvest treatments of Camellia oleifera fruit 〔without treatment (F8), composting treatment(FC) , sun dried without shell (NS), and sun dried with shell(WS)〕 for 10 d and 30 d, and relevant software was used to analyze the metabolites. The results showed that a total of 1 107 characteristic metabolites were identified, with 763 and 344 metabolites identified in the positive and negative ion modes, respectively. The main metabolites were lipids and lipid-like molecules( 277 kinds), phenylpropanoids and polykettides (193 kinds), organic acids and derivatives(172 kinds), benzenoids (97 kinds), organoheterocyclic compounds (94 kinds), organic oxygen compounds (84 kinds), nucleosides, nucleotides and analogues(33 kinds). By comparison of the unsaturated fatty acid of differential metabolites, six unsaturated fatty acids, namely linoleic acid, cis-9-hexadecenoic acid, 8 (9) - epoxy-5Z, 11Z, 14Z-eicosatrienoic acid, linolenic acid, ricinolic acid, 9,10-epoxy-octadecenoic acid, were detected in 30 d (NS2) and 10 d (NS1) of the sun dried without shell treatment, and their expressions were up-regulated. The enrichment analysis of differential metabolites results showed that the main metabolic pathways included phenylalanine metabolism, flavonoid biosynthesis, glycerol phospholipid metabolism, glycerol ester metabolism, zeatin biosynthesis, plant secondary metabolites biosynthesis, glutathione metabolism, amino acid biosynthesis, and secondary metabolites biosynthesis. Overall, the best postharvest treatment of Camellia oleifera fruit is sun dried without shell treatment for 30 d.
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