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| Preparation of zero trans and low saturated margarine base oil by domestic Pichia pastoris lipase catalyzed transesterification |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230094 |
| KeyWord:domestic lipase low saturated margarine base oil enzymatic transesterification high oleic acid sunflower seed oil |
| FundProject:广东省重点领域研发计划项目(2022B0202010003)；小麦和玉米深加工国家工程研究中心开放课题（NL2022007） |
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| Abstract: |
| Aiming to prepare zero trans and low saturated margarine base oil at low cost, using high oleic acid sunflower seed oil and palm stearin as raw materials, 9 lipases from domestic and foreign as catalysts, margarine base oil was prepared by transesterification, and an economical and efficient lipase based on solid fat content (SFC) and sliding melting point as inspection indicators was selected. Then single factor experiment and response surface methodology were used to optimize the reaction conditions of enzymatic transesterification with the selected lipase as catalyst, and the fatty acid composition, triglyceride(TAG) composition, and thermodynamic properties of the base oil before and after enzymatic transesterification were compared. The results showed that the domestic Pichia pastoris lipase was inexpensive and had high catalytic activity. The optimal conditions for enzymatic transesterification were enzyme addition amount 17%, reaction time 28 min and reaction temperature 48 ℃. The base oil was prepared by 10× magnification experiment under the optimal conditions, and its SFC at 5 ℃ and 25 ℃ were 22.35% and 11.67%, respectively. Compared with the oil before transesterification, the fatty acid composition of transesterification products had no significant change, TAG transformed from U3 and S3 to S2U and SU2, the transesterification rate was 76.40%, the content of TAG at middle melting point increased, the content of TAG at high melting point and low melting point decreased, the heat for melting decreased, and the taste was smoother. Therefore, domestic Pichia pastoris lipase is inexpensive and suitable as a catalyst for the preparation of margarine base oil by enzymatic transesterification. |
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