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| Cholesterol-lowering effect of flaxseed protein hydrolysate peptides |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230224 |
| KeyWord:flaxseed protein enzymatic peptides mice cholesterol-lowering effect |
| FundProject:内蒙古自治区科技计划项目（2020GG0064）；国家自然科学基金地区科学基金（31860423） |
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| Abstract: |
| To provide a reference for the deep processing and utilization of flaxseed protein, the flaxseed protein was prepared by alkali-soluble and acid-precipitation method from low-temperature pressed flaxseed cake, and then the flaxseed peptides were prepared by enzymatic hydrolysis. Ultrafiltration membranes were used to isolate flaxseed peptides with the highest cholesterol-lowering activity in vitro, and animal experiments were conducted to explore its cholesterol-lowering effects. The results showed that when the enzymatic hydrolysis time was 4 h, the cholesterol micelle solubility inhibition rate of flaxseed peptide was the highest (47.57%), and the cholesterol micelle solubility inhibition rate of the ultrafiltration fraction with a molecular weight of no more than 3 kDa was the highest (70.96%). Compared with the high-cholesterol diet group, the ultrafiltration fraction(≤3 kDa ) could increase the food intake and body weight of mice, reduce the serum total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) levels, increase the serum high-density lipoprotein cholesterol (HDL-C) level, reduce liver index and TC and TG levels in the liver, improve the degree of hepatic steatosis, increase fecal total bile acid (TBA) excretion, and reduce the oxidative stress caused by high-cholesterol diet by reducing malondialdehyde (MDA) level and increasing superoxide dismutase (SOD) activity. In conclusion, the ultrafiltration fraction (≤3 kDa )of flaxseed peptides has a certain cholesterol-lowering effect, and is expected to become a new cholesterol-lowering functional component. |
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