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| Antioxidant activity of zein peptides modified by TGase-catalyzed chitosan oligosaccharides glycosylation |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230228 |
| KeyWord:zein peptides chitosan oligosaccharides zein glycopeptides in vitro digestion antioxidant activity |
| FundProject:黑龙江省玉米深加工理论与技术重点实验室开放课题（SPKF202005） |
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| Abstract: |
| To further enhance the antioxidant activity of the zein peptides, the active peptides from zein have been used as raw material, and then zein glycopeptides were prepared by transglutaminase(TGase)-induced chitosan oligosaccharides (molecular weight 1 500 Da) covalent binding onto zein peptides. The effect of chitosan oligosaccharides modification on the antioxidant activities of zein peptides was studied. In addition, zein glycopeptides were simulated by gastrointestinal digestion in vitro to characterize its antioxidant potential in vivo. The results showed that the covalent binding of chitosan oligosaccharides catalyzed by TGase significantly increased peptide content and in vitro digestibility of zein peptides. At the mass concentration of 2.0 mg/mL, the DPPH, hydroxyl and superoxide anion radicals scavenging rates, Fe2+-chelating capacity and reducing capacity of TGase modified zein peptides increased, also the DPPH, hydroxyl radicals scavenging rates and reducing capacity of zein glycopeptides after in vitro digestion increased, while the Fe2+-chelating capacity and the superoxide anion radical scavenging rate decreased. In conclusion, TGase-modiated chitosan glycosylation significantly improves the antioxidant activities of zein peptides, and in vitro digestion can promote the release of the antioxidant activities of zein glycopeptides (except Fe2+-chelating capacity and superoxide anion radical scavenging ability). |
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